Description

3,3'-Dithiopropionyl-1-Sulfosuccinimidyl 1'-Glycyl-Tyrosyl Cholamine is a specialized heterobifunctional crosslinking reagent designed for advanced bioconjugation applications. This compound matters because it provides a unique combination of a cleavable disulfide bond and an amine-reactive NHS ester, enabling the controlled formation of reversible linkages between biomolecules. It is primarily needed by researchers and manufacturers in the fields of biopharmaceutical development, diagnostic assay production, and advanced materials science for creating sophisticated drug delivery systems, protein-protein conjugates, and immobilized enzyme matrices.

Application

• Bioconjugation and Crosslinking: For creating reversible linkages between proteins, peptides, and other amine-containing biomolecules via its NHS ester and cleavable disulfide bridge.
• Drug Delivery System Development: As a key reagent for constructing targeted, stimuli-responsive drug carriers where the disulfide bond is cleaved in the reducing intracellular environment.
• Diagnostic Reagent Manufacturing: In the production of enzyme-labeled antibodies and other detection conjugates for immunoassays (ELISA, lateral flow tests).
• Affinity Chromatography Ligand Immobilization: For attaching proteins or peptides to solid supports like agarose beads, allowing for reversible capture and elution.
• Surface Functionalization: To modify gold nanoparticles, biosensor chips, or other material surfaces with bioactive molecules for research and diagnostic platforms.
• Proteomics Research: As a tool for studying protein interactions, dynamics, and complex formation through reversible crosslinking strategies.

Basic Information

Product Name	3,3'-Dithiopropionyl-1-Sulfosuccinimidyl 1'-Glycyl-Tyrosyl Cholamine
CAS No.	119463-04-6
Molecular Formula	C31H43N5O12S3
Molecular Weight	781.89 g/mol
Synonyms	DTSSP-Gly-Tyr-Cholamine; 3,3'-Dithiobis(sulfosuccinimidyl propionate) Gly-Tyr-Cholamine derivative; Sulfo-LC-SPDP-Gly-Tyr-Cholamine; Heterobifunctional Crosslinker 119463-04-6; NHS ester disulfide crosslinker; N-Succinimidyl 3-(2-pyridyldithio)propionate-Gly-Tyr-Cholamine analog; SPDP derivative with Glycyl-Tyrosyl Cholamine; Water-soluble cleavable crosslinking reagent
EINECS	Contact for details

Quality Control

Our 3,3'-Dithiopropionyl-1-Sulfosuccinimidyl 1'-Glycyl-Tyrosyl Cholamine is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity assessment, NMR and MS for structural confirmation, and functional activity validation in standard conjugation assays. Certificates of Analysis (COA) detailing purity, identity, and performance are available upon request to ensure compliance with your research and development protocols.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a desiccated environment. This compound is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake. For long-term stability, store under an inert atmosphere such as argon or nitrogen.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Retention time corresponds to reference standard
Identification (MS)	Mass spectrum consistent with molecular structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 2.0%
Functional Activity (Conjugation Assay)	≥ 90% of theoretical coupling efficiency

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.