Description

Boc-Ala-Gly-Pro-Arg-7-Amino-4-Methylcoumarin is a specialized fluorogenic peptide substrate designed for protease activity assays. This compound is of significant value in biochemical research and pharmaceutical development for its role in detecting and quantifying enzyme activity with high sensitivity. It is primarily needed by research institutions, diagnostic kit manufacturers, and pharmaceutical companies engaged in protease inhibitor screening and enzyme kinetics studies.

Application

• Protease Activity Assays: A key fluorogenic substrate for measuring the activity of trypsin, thrombin, and related serine proteases in vitro.
• High-Throughput Screening (HTS): Used in automated platforms for the discovery and evaluation of novel protease inhibitors as potential drug candidates.
• Biochemical Research: Essential for studying enzyme kinetics, specificity, and catalytic mechanisms in academic and industrial laboratories.
• Diagnostic Development: Serves as a critical component in the development of clinical diagnostic tests for conditions related to abnormal protease activity.
• Quality Control in Biologics: Employed to monitor and ensure the absence of residual protease activity in biopharmaceutical manufacturing processes.

Basic Information

Product Name	Boc-Ala-Gly-Pro-Arg-7-Amino-4-Methylcoumarin
CAS No.	118850-78-5
Molecular Formula	C₃₄H₅₀N₁₀O₉
Molecular Weight	746.83 g/mol
Synonyms	Boc-Ala-Gly-Pro-Arg-AMC; Boc-Ala-Gly-Pro-Arg-7-Amido-4-methylcoumarin; t-Butyloxycarbonyl-Ala-Gly-Pro-Arg-4-Methylcoumaryl-7-amide; BOC-A-G-P-R-AMC; Fluorogenic Substrate for Trypsin; Protease Substrate 118850-78-5; AMC-labeled Peptide Substrate
EINECS	Contact for details

Quality Control

Our Boc-Ala-Gly-Pro-Arg-7-Amino-4-Methylcoumarin is produced under strict quality management systems to ensure batch-to-batch consistency and high purity for sensitive research applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation. Certificates of Analysis (COA) detailing all specifications are available upon request to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a freezer. Due to its hygroscopic (moisture-sensitive) nature, the container should be allowed to reach room temperature before opening to prevent condensation and moisture uptake. For long-term stability, store under inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Identification (Mass Spec)	Conforms
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water
Functional Activity	Conforms to reference standard in enzyme assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.