Description

24,25-Dihydroxy Vitamin D2-D3 is a key metabolite in the vitamin D metabolic pathway, representing a critical intermediate for research and development. This compound is essential for studying the biological activity and regulatory mechanisms of vitamin D beyond its classical hormonal forms. It is primarily utilized by researchers and manufacturers in the pharmaceutical, nutraceutical, and life science sectors for analytical standards, biochemical research, and as a reference material.

Application

• Biochemical Research: Used as a reference standard and intermediate in studies investigating the metabolism, catabolism, and biological functions of vitamin D.
• Pharmaceutical Development: Serves as a critical intermediate or analytical standard in the synthesis and quality control of novel vitamin D analogs and metabolites.
• Clinical Diagnostics: Employed as a calibrant or control in the development of assays (e.g., LC-MS/MS) for quantifying vitamin D metabolites in biological samples.
• Nutraceutical Analysis: Acts as a high-purity reference material for the accurate analysis and standardization of complex vitamin D formulations.
• Metabolic Pathway Studies: Essential for in vitro and in vivo research to elucidate the specific roles of 24,25-dihydroxy metabolites in calcium homeostasis and other physiological processes.

Basic Information

Product Name	24,25-Dihydroxy Vitamin D2-D3
CAS No.	118584-50-2
Molecular Formula	C27H44O3 / C28H46O3 (Isomeric mixture)
Molecular Weight	416.64 g/mol (D3 form) / 430.66 g/mol (D2 form)
Synonyms	24,25-Dihydroxycholecalciferol; 24,25-Dihydroxyvitamin D3; 24,25-(OH)2D3; 24,25-Dihydroxyergocalciferol; 24R,25-Dihydroxyvitamin D3; (24R)-24,25-Dihydroxyvitamin D3; 24,25-Dihydroxy Vitamin D Metabolite; Secalciferol (related metabolite)
EINECS	Contact for details

Quality Control

Our 24,25-Dihydroxy Vitamin D2-D3 is produced and handled under strict quality management protocols. Each batch undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation (IR, NMR), to ensure it meets the high standards required for research and development. A detailed Certificate of Analysis (COA) is provided with each shipment, documenting batch-specific results.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C (refrigerated) or as indicated on the label or COA. This product is hygroscopic (moisture-sensitive) and light-sensitive; therefore, containers must be kept sealed under an inert atmosphere when possible and allowed to reach room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/IR)	Conforms to reference standard
Assay (HPLC)	≥ 95.0% (Total of D2 and D3 isomers)
Water Content (KF)	≤ 2.0%
Related Substances (HPLC)	Individual unknown impurity ≤ 1.0% Total impurities ≤ 5.0%
Solubility	Soluble in ethanol, DMSO; sparingly soluble in aqueous buffers

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.