Description

Cytidine-5'-o-(1-Thiotriphosphate), Rp-Isomer Sodium Salt is a chemically modified nucleotide analog where a non-bridging oxygen atom in the α-phosphate group is replaced by sulfur, specifically in the Rp diastereomeric configuration. This modification renders it resistant to hydrolysis by many phosphatases, making it an essential tool for mechanistic studies in enzymology and molecular biology. It is primarily utilized by researchers in pharmaceutical R&D, diagnostic assay development, and academic institutions focusing on nucleic acid biochemistry and kinase signaling pathways.

Application

• Enzymology Research: Used as a substrate analog to study the mechanism of ATP/GTP-utilizing enzymes, particularly polymerases, kinases, and GTPases, due to its phosphorothioate linkage.
• Nucleic Acid Labeling: Employed in the synthesis of thio-modified RNA or DNA probes for structural studies and to trace phosphorylation events.
• Kinase Activity Assays: Serves as a substrate in high-throughput screening (HTS) assays to identify and characterize kinase inhibitors for drug discovery.
• Structural Biology: Aids in X-ray crystallography and NMR studies to determine enzyme active site structures and binding interactions.
• Diagnostic Development: Used in the development of sensitive diagnostic kits that rely on nucleotide incorporation, such as next-generation sequencing (NGS) platforms.
• Biochemical Tool: Acts as a non-hydrolyzable or slow-turnover analog to trap reaction intermediates in multi-step enzymatic reactions.

Basic Information

Product Name	Cytidine-5'-o-(1-Thiotriphosphate), Rp-Isomer Sodium Salt
CAS No.	118374-59-7
Molecular Formula	C9H14N3O13P3S • xNa+
Molecular Weight	Approx. 527.1 (Free acid) - Contact for details on sodium salt form.
Synonyms	CTPαS, Rp isomer; Cytidine 5'-(α-thiotriphosphate), Rp diastereomer; Rp-CTPαS; 5'-CTP-α-S, Rp isomer; Cytidine-5'-triphosphate, 1-thio-, Rp-isomer sodium salt; Sp-1-Thio-CTP (common mislabeling for Rp); Adenosine 5'-(γ-thiotriphosphate) analog (class); Nucleoside phosphorothioate triphosphate
EINECS	Contact for details.

Quality Control

Our Cytidine-5'-o-(1-Thiotriphosphate), Rp-Isomer Sodium Salt is produced under strict quality systems suitable for research and diagnostic use. Each batch undergoes rigorous analytical testing to ensure high diastereomeric purity and chemical integrity. Certificates of Analysis (COA) are provided, detailing parameters such as purity (by HPLC), identity confirmation, and moisture content. We adhere to ISO 9001 guidelines, and custom synthesis to meet specific purity or packaging requirements is available.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a desiccated environment. This product is hygroscopic (moisture-sensitive). For long-term stability, store under inert atmosphere or in aliquots to minimize freeze-thaw cycles. Allow the sealed vial to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white powder or solid
Identification (HPLC)	Conforms to reference standard
Purity (HPLC)	≥ 95% (Rp-isomer)
Diastereomeric Excess (de)	≥ 98% (Rp)
Water Content (KF)	≤ 5.0 %
Biological Activity	Suitable for enzyme assays

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.