Description

Lys-Ala 4-Methoxy-β-Naphthylamide Dihydrochloride is a specialized chromogenic peptide substrate used as a critical reagent in enzymatic assays. This compound is valued for its high sensitivity and specificity in detecting and quantifying protease activity, particularly from enzymes like cathepsins. It is essential for researchers and quality control laboratories in the pharmaceutical, biotechnology, and diagnostic sectors who require reliable and consistent biochemical tools.

Application

• Protease Activity Assays: Primary substrate for the fluorometric or colorimetric detection and kinetic study of lysosomal cysteine proteases, such as cathepsin B and L.
• Biochemical Research: Used in enzyme characterization, inhibitor screening, and mechanistic studies within academic and industrial R&D settings.
• Diagnostic Reagent Manufacturing: A key component in the formulation of test kits for clinical diagnostics and pathological analysis.
• Pharmaceutical Development: Employed in drug discovery pipelines to evaluate the efficacy of novel protease-targeting therapeutic compounds.
• Cell Biology Studies: Utilized to measure protease activity in cell lysates and tissue samples, aiding in studies of apoptosis, protein turnover, and disease mechanisms.

Basic Information

Product Name	Lys-Ala 4-Methoxy-β-Naphthylamide Dihydrochloride
CAS No.	118357-26-9
Molecular Formula	C₂₅H₃₄Cl₂N₄O₅
Molecular Weight	541.47 g/mol
Synonyms	L-Alanyl-L-lysine 4-methoxy-2-naphthylamide dihydrochloride; Nα-(4-Methoxy-2-naphthoyl)-L-lysyl-L-alaninamide dihydrochloride; Ala-Lys-4MβNA·2HCl; Cathepsin Substrate; L-Lys-L-Ala-4-methoxy-β-naphthylamide dihydrochloride; Lys-Ala-4MβNA; N-(4-Methoxy-2-naphthoyl)-Lys-Ala-NH₂·2HCl
EINECS	Contact for details

Quality Control

Our Lys-Ala 4-Methoxy-β-Naphthylamide Dihydrochloride is manufactured under strict quality systems to ensure batch-to-batch consistency and high purity suitable for sensitive biochemical applications. Each lot is supported by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and performance in standard assay conditions. We adhere to relevant quality guidelines to support our customers in research and regulated environments.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. The product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation from atmospheric moisture.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/IR)	Conforms to reference standard
Assay (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Solubility	Clear, colorless solution in DMSO or buffer
Performance in Assay	Conforms to reference activity

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.