Description

n-p-Tosyl-Gly-Pro-Arg 7-Amido-4-Methylcoumarin Hydrochloride is a specialized fluorogenic peptide substrate designed for the sensitive detection and quantification of trypsin-like serine proteases. This compound matters for researchers and quality control labs requiring precise, high-throughput enzymatic activity assays. It is primarily needed by the pharmaceutical, biotechnology, and diagnostic industries for applications in drug discovery, proteomics research, and enzyme characterization.

Application

• Protease Activity Assays: A key fluorogenic substrate for measuring the activity of trypsin, thrombin, plasmin, and other related serine proteases in biochemical research.
• High-Throughput Screening (HTS): Enables rapid screening of enzyme inhibitors or activators in drug discovery platforms for cardiovascular and inflammatory diseases.
• Diagnostic Kit Development: Used as a critical component in the formulation of diagnostic kits for clinical analysis of blood coagulation and fibrinolysis pathways.
• Enzyme Kinetics & Characterization: Essential for detailed kinetic studies (Km, Vmax, kcat) to characterize novel proteases or enzyme mutants.
• Cell Biology & Proteomics: Employed in cellular assays to monitor protease activity in real-time within live cells or tissue extracts.
• Quality Control in Biologics: Used in QC labs to monitor and validate the activity and consistency of enzyme-based biopharmaceutical products.

Basic Information

Product Name	n-p-Tosyl-Gly-Pro-Arg 7-Amido-4-Methylcoumarin Hydrochloride
CAS No.	117961-27-0
Molecular Formula	C31H38N8O7S • HCl
Molecular Weight	711.21 g/mol (Free base: 675.75 g/mol)
Synonyms	Tos-Gly-Pro-Arg-AMC • HCl; Gly-Pro-Arg-7-amido-4-methylcoumarin Tosylate; TGP-AMC; Trypsin Substrate I (Fluorogenic); Boc-Gly-Pro-Arg-AMC related compound; AMC-based trypsin substrate; H-Gly-Pro-Arg-AMC • Tosylate • HCl; 7-Amido-4-methylcoumarin conjugate of Tos-Gly-Pro-Arg
EINECS	Contact for details

Quality Control

Our n-p-Tosyl-Gly-Pro-Arg 7-Amido-4-Methylcoumarin Hydrochloride is manufactured under strict quality systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive research and diagnostic applications. Certificates of Analysis (COA) with detailed chromatographic data are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive; allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Identification (Mass Spec)	Conforms
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in water
Fluorogenic Activity	Functional activity confirmed vs. reference standard

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.