Description

Dl-Val-Leu-Arg p-Nitroanilide Acetate Salt is a synthetic tripeptide derivative of p-nitroaniline, widely utilized as a chromogenic substrate for the study and quantification of serine protease activity. Its core value lies in providing a reliable, colorimetric readout for enzymatic assays, enabling precise biochemical research and diagnostic development. This compound is essential for researchers and manufacturers in the fields of pharmaceutical R&D, clinical diagnostics, and biochemical research, particularly for investigating enzymes like thrombin, trypsin, and related proteases.

Application

• Protease Activity Assays: Primary chromogenic substrate for the kinetic study and quantification of serine proteases, including thrombin, trypsin, and plasmin.
• Pharmaceutical Research: Used in high-throughput screening (HTS) platforms to identify and characterize potential protease inhibitors for drug discovery.
• Diagnostic Kit Manufacturing: A key component in the formulation of clinical diagnostic kits for measuring coagulation factors and other protease-related biomarkers.
• Enzyme Characterization: Essential for determining kinetic parameters (Km, Vmax) and specificity constants of purified proteases in biochemical research.
• Quality Control in Biologics: Employed to monitor and validate the activity and purity of enzyme preparations used in therapeutic production.
• Academic & Institutional Research: A standard reagent in university and institutional labs for teaching and advanced research in enzymology and cell signaling pathways.

Basic Information

Item	Detail
Product Name	Dl-Val-Leu-Arg p-Nitroanilide Acetate Salt
CAS No.	117961-23-6
Molecular Formula	C24H39N9O7 • xC2H4O2
Molecular Weight	Contact for details
Synonyms	H-DL-Val-Leu-Arg-pNA acetate salt; D-Valyl-L-leucyl-L-arginine 4-nitroanilide acetate; Val-Leu-Arg pNA; Thrombin Substrate I (pNA-based); S-2366 (Chromogenic Substrate); Tosyl-Gly-Pro-Arg p-nitroanilide acetate salt analog; Boc-Val-Pro-Arg pNA related substrate; Protease chromogenic substrate 117961-23-6
EINECS	Contact for details

Quality Control

Our Dl-Val-Leu-Arg p-Nitroanilide Acetate Salt is produced and tested under a strict quality management system to ensure batch-to-batch consistency and high performance in sensitive assays. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and functional activity. We adhere to relevant industry guidelines for research-grade biochemicals to guarantee reliability for your critical applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. The product is hygroscopic (moisture-sensitive); allow the sealed vial to equilibrate to room temperature before opening to minimize moisture uptake. For long-term stability, store desiccated.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/MS)	Conforms to structure
Assay (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in water, DMSO
Functional Activity	Conforms to reference standard

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.