Description

Dl-Val-Leu-Arg p-Nitroanilide Acetate Salt is a synthetic tripeptide chromogenic substrate designed for the specific detection and quantification of serine protease activity. This compound matters for researchers and quality control labs requiring precise, reliable, and reproducible enzymatic assays. It is primarily needed by the pharmaceutical, diagnostic, and biochemical research industries for applications in drug discovery, enzyme characterization, and diagnostic kit development.

Application

• Protease Activity Assays: As a chromogenic substrate for the kinetic study and quantification of trypsin, thrombin, plasmin, and related serine proteases.
• Pharmaceutical R&D: Used in high-throughput screening (HTS) platforms to identify and characterize novel protease inhibitors for therapeutic development.
• Diagnostic Manufacturing: A key component in the formulation of clinical diagnostic kits for measuring coagulation factors and other protease levels in blood plasma.
• Biochemical Research: Essential tool for investigating protease specificity, enzyme kinetics, and catalytic mechanisms in academic and industrial laboratories.
• Quality Control (QC): Employed as a reference standard in QC testing for biopharmaceuticals where protease activity must be monitored or controlled.

Basic Information

Product Name	Dl-Val-Leu-Arg p-Nitroanilide Acetate Salt
CAS No.	117961-22-5
Molecular Formula	C24H39N9O7 • xC2H4O2
Molecular Weight	565.63 g/mol (Free base)
Synonyms	Val-Leu-Arg pNA; H-DL-Val-Leu-Arg-p-nitroanilide acetate; VLR-pNA; N-(p-Nitroanilino)-DL-valyl-L-leucyl-L-arginine acetate; DL-Valyl-L-leucyl-L-arginine 4-nitroanilide acetate salt; Chromogenic Substrate for Trypsin; Boc-Val-Leu-Arg-pNA (related precursor); Protease Substrate 117961-22-5
EINECS	Contact for details

Quality Control

Our Dl-Val-Leu-Arg p-Nitroanilide Acetate Salt is manufactured under strict quality systems to ensure batch-to-batch consistency and high performance in sensitive enzymatic assays. Each lot undergoes rigorous analytical testing, including HPLC for purity and identity confirmation. Certificates of Analysis (COA) detailing purity, assay, and related substances are available upon request to support your regulatory and research documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive; prolonged exposure to moisture, heat, or light may lead to decomposition and reduced performance. For long-term storage, consider desiccant and under inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Assay (Enzymatic)	≥ 95% of theoretical activity
Solubility	Soluble in DMSO, DMF; slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.