Description

n-tert-Butoxycarbonyl-Alanyl-Alanyl-Prolyl-Aspartyl-4-Nitroanilide is a specialized synthetic peptide derivative designed for advanced biochemical research and pharmaceutical development. This compound serves as a critical chromogenic substrate for the study and detection of specific proteolytic enzyme activity, particularly in high-throughput screening and diagnostic assay development. It is an essential tool for researchers and manufacturers in the fields of enzymology, drug discovery, and in vitro diagnostics, where precise and reliable substrates are required to monitor enzyme kinetics and inhibition.

Application

• Enzyme Substrate for Protease Research: Primarily used as a chromogenic substrate for the study of serine proteases and other specific peptidases, enabling the measurement of enzymatic activity through the release of p-nitroaniline.
• High-Throughput Screening (HTS): Employed in pharmaceutical and biotechnology labs for screening libraries of compounds to identify potential protease inhibitors as drug candidates.
• Diagnostic Assay Development: Integral in the development of colorimetric diagnostic kits for detecting and quantifying specific enzyme levels in clinical and research samples.
• Biochemical Mechanism Studies: Utilized to investigate the catalytic mechanisms, specificity, and kinetics of target enzymes in purified systems.
• Quality Control in Biologics: Applied in the quality control testing of therapeutic proteins to assess the presence and activity of contaminating proteases.

Basic Information

Product Name	n-tert-Butoxycarbonyl-Alanyl-Alanyl-Prolyl-Aspartyl-4-Nitroanilide
CAS No.	117722-96-0
Molecular Formula	C27H38N6O10
Molecular Weight	606.63 g/mol
Synonyms	Boc-Ala-Ala-Pro-Asp-pNA; Boc-AAPD-pNA; t-Butoxycarbonyl-Alanyl-Alanyl-Prolyl-Aspartyl 4-Nitroanilide; Substrate for Protease; Chromogenic Peptide Substrate AAPD-pNA; Ala-Ala-Pro-Asp p-Nitroanilide; N-Boc-AAPD-pNA; Protease Substrate IV
EINECS	Contact for details

Quality Control

Our n-tert-Butoxycarbonyl-Alanyl-Alanyl-Prolyl-Aspartyl-4-Nitroanilide is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive research applications. Certificates of Analysis (COA) detailing purity, assay, and related substances are provided with every shipment to guarantee traceability and consistency for your critical work.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. Due to its hygroscopic nature, allow the vial to equilibrate to room temperature in a desiccator before opening to prevent moisture uptake. For long-term storage, consider under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (Mass Spectrometry)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Assay	≥ 98.0% (on dried basis)
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.