Description

tert-Butoxycarbonyl-Alanyl-Alanyl-Prolyl-Glutamyl-4-Nitroanilide is a synthetic peptide derivative containing a chromogenic nitroanilide group, widely used as a specialized substrate in enzymatic research. Its core value lies in providing a precise and measurable tool for studying protease activity, particularly for enzymes like dipeptidyl peptidase IV (DPP-IV). This compound is essential for researchers and manufacturers in the pharmaceutical, biotechnology, and diagnostic reagent sectors who require high-purity biochemical tools for assay development and enzyme characterization.

Application

• Enzymology Research: Primary substrate for the kinetic study and inhibition profiling of serine proteases, including DPP-IV and related enzymes.
• Pharmaceutical Screening: Used in high-throughput screening (HTS) assays to identify and evaluate potential therapeutic inhibitors for metabolic and inflammatory diseases.
• Diagnostic Reagent Manufacturing: A key component in the formulation of clinical diagnostic kits for measuring specific protease activity in biological samples.
• Biochemical Assay Development: Serves as a model chromogenic substrate for developing and optimizing new in vitro enzymatic assay protocols.
• Academic Research: Utilized in university and institutional labs for fundamental research on peptide hydrolysis mechanisms and enzyme specificity.

Basic Information

Product Name	tert-Butoxycarbonyl-Alanyl-Alanyl-Prolyl-Glutamyl-4-Nitroanilide
CAS No.	117722-95-9
Molecular Formula	C31H45N7O10
Molecular Weight	675.73 g/mol
Synonyms	Boc-Ala-Ala-Pro-Glu-pNA; Boc-AAPG-pNA; H-Glu(pNA)-OH containing peptide; DPP-IV Substrate; Ala-Ala-Pro-Glu-4-Nitroanilide, Boc-protected; 4-Nitroanilide of Boc-AAPG-OH; N-α-(tert-Butoxycarbonyl)-L-alanyl-L-alanyl-L-prolyl-L-glutamyl 4-nitroanilide
EINECS	Contact for details

Quality Control

Every batch of our tert-Butoxycarbonyl-Alanyl-Alanyl-Prolyl-Glutamyl-4-Nitroanilide is produced and tested under stringent quality management systems. We guarantee high purity and batch-to-batch consistency suitable for sensitive research and diagnostic applications. Certificates of Analysis (COA) detailing purity, identity, and impurity profiles by HPLC are provided with each shipment to ensure full traceability and compliance with your research protocols.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive) and should be allowed to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis. For long-term stability, store under inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.