Description

4-(Trifluoromethyl)Umbelliferyl-β-D-Galactopyranoside is a specialized fluorogenic substrate designed for high-sensitivity enzymatic assays. This compound matters because it provides a reliable and highly detectable signal upon cleavage by β-galactosidase, making it indispensable for precise biochemical research and diagnostics. It is primarily needed by biotechnology, pharmaceutical, and diagnostic kit manufacturers for applications in enzyme kinetics, high-throughput screening, and molecular biology research.

Application

• Fluorogenic Substrate for β-Galactosidase: Used as a key reagent in enzymatic assays to detect and quantify β-galactosidase activity with high sensitivity.
• Molecular Biology Research: Essential for reporter gene assays (e.g., lacZ gene expression) in genetic engineering and cellular biology studies.
• Diagnostic Kit Manufacturing: Incorporated into diagnostic kits for the detection of enzymatic markers associated with certain genetic disorders or bacterial identification (e.g., E. coli).
• High-Throughput Screening (HTS): Utilized in drug discovery platforms to identify inhibitors or activators of β-galactosidase in a rapid, automated format.
• Biochemical Research: Applied in enzyme kinetics studies to determine kinetic parameters like Km and Vmax for β-galactosidase.
• Cell-Based Assays: Used in live-cell imaging and flow cytometry to monitor gene expression or cell signaling pathways linked to β-galactosidase reporters.

Basic Information

Item	Detail
Product Name	4-(Trifluoromethyl)Umbelliferyl-β-D-Galactopyranoside
CAS No.	117153-55-6
Molecular Formula	C₁₆H₁₅F₃O₈
Molecular Weight	392.28 g/mol
Synonyms	4-Trifluoromethylumbelliferyl β-D-galactopyranoside; 4-Methylumbelliferyl-β-D-galactopyranoside trifluoromethyl analog; TFMU-β-D-Gal; 4-(Trifluoromethyl)-7-hydroxycoumarin β-D-galactopyranoside; β-Galactosidase substrate (trifluoromethyl); Galactopyranosyloxy-7-(trifluoromethyl)coumarin; 117153-55-6; 4-Trifluoromethyl-7-β-D-galactopyranosyloxycoumarin
EINECS	Contact for details

Quality Control

Our 4-(Trifluoromethyl)Umbelliferyl-β-D-Galactopyranoside is manufactured under strict quality systems to ensure batch-to-batch consistency and high purity suitable for sensitive research and diagnostic applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and performance in standard enzymatic assays. We adhere to relevant quality guidelines to support our customers' compliance needs in regulated environments.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive; prolonged exposure to moisture, elevated temperature, or light should be avoided to maintain stability and performance.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/IR)	Conforms to reference standard
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Specific Rotation	Contact for details
Fluorogenic Activity	Conforms to performance standard
Solubility	Soluble in DMSO, DMF; slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.