Description

tert-Butyloxycarbonyl-Leucyl-Glycyl-Arginine-4-Trifluoromethylcoumarin-7-Amide is a specialized fluorogenic peptide substrate designed for high-sensitivity enzymatic assays. This compound is critically important for biochemical research and pharmaceutical development, serving as a key tool for the detection and quantification of protease activity. It is primarily needed by researchers and quality control laboratories in the life sciences, pharmaceutical manufacturing, and diagnostic development sectors.

Application

• Protease Activity Assays: Used as a highly sensitive fluorogenic substrate for measuring the activity of trypsin-like proteases, including thrombin, plasmin, and urokinase-type plasminogen activator (uPA).
• Drug Discovery Screening: Employed in high-throughput screening (HTS) platforms to identify and characterize novel protease inhibitors for therapeutic development.
• Biochemical Research: A vital reagent for studying protease kinetics, enzyme specificity, and catalytic mechanisms in academic and industrial research settings.
• Diagnostic Development: Utilized in the development of clinical diagnostic kits for conditions related to abnormal protease activity, such as coagulation disorders or cancer metastasis.
• Quality Control in Biologics: Applied in the quality control of biopharmaceuticals, particularly for monitoring residual host cell protease activity during downstream purification processes.

Basic Information

Product Name	tert-Butyloxycarbonyl-Leucyl-Glycyl-Arginine-4-Trifluoromethylcoumarin-7-Amide
CAS No.	117141-39-6
Molecular Formula	C₃₃H₄₈F₃N₉O₈
Molecular Weight	779.80 g/mol
Synonyms	Boc-Leu-Gly-Arg-AFC; Boc-Leu-Gly-Arg-Amc; AFC Substrate for Protease; t-Butyloxycarbonyl-Leucyl-Glycyl-Arginine-7-Amido-4-Trifluoromethylcoumarin; Fluorogenic Protease Substrate; 7-Amido-4-trifluoromethylcoumarin-Leu-Gly-Arg; Boc-LGR-AFC; t-BOC-Leu-Gly-Arg-AFC
EINECS	Contact for details

Quality Control

Our tert-Butyloxycarbonyl-Leucyl-Glycyl-Arginine-4-Trifluoromethylcoumarin-7-Amide is manufactured under strict quality systems to ensure batch-to-batch consistency and high performance in sensitive assays. Each lot undergoes rigorous analytical testing, including HPLC for purity and spectroscopic identification. Certificates of Analysis (COA) detailing purity, identity, and performance data are provided with every shipment to support your research and compliance needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (Mass Spec)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 2.0%
Fluorogenic Activity	Functional in standard assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.