Description

5-(2,5-Dioxopyrrolidin-1-yl)oxy-5-oxopentanoic acid is a high-purity, heterobifunctional crosslinking reagent featuring an activated NHS ester and a carboxylic acid group. This compound is essential for creating stable amide bonds with primary amines while providing a free carboxyl group for further conjugation or surface modification. It is primarily required by researchers and manufacturers in the pharmaceutical, bioconjugation, and advanced materials sectors for developing targeted drug delivery systems, diagnostic assays, and functionalized polymers.

Application

• Bioconjugation and Protein Labeling: As a key linker for covalently attaching proteins, peptides, or antibodies to other biomolecules, nanoparticles, or solid surfaces via its NHS ester and carboxylic acid functionalities.
• Polymer and Biomaterial Functionalization: For introducing reactive carboxyl groups into polymer backbones or hydrogel matrices to enable further chemical modification and crosslinking.
• Diagnostic Reagent Synthesis: In the development of enzyme-linked immunosorbent assay (ELISA) kits, lateral flow assays, and other diagnostic tools where stable, site-specific labeling is critical.
• Drug Delivery System Development: For constructing antibody-drug conjugates (ADCs) or other targeted therapeutic platforms by linking drug payloads to targeting ligands.
• Surface Chemistry and Immobilization: To create functionalized surfaces on chips, beads, or sensors for biosensing and microarray applications.
• Organic Synthesis Intermediate: Serves as a versatile building block in medicinal chemistry for synthesizing more complex molecules with specific spacer arms and reactivity.

Basic Information

Product Name	5-(2,5-Dioxopyrrolidin-1-yl)oxy-5-oxopentanoic Acid
CAS No.	116920-04-8
Molecular Formula	C9H11NO7
Molecular Weight	245.19 g/mol
Synonyms	NHS-Glutamic Acid; 5-[(2,5-Dioxo-1-pyrrolidinyl)oxy]-5-oxopentanoic Acid; Glutamic Acid 5-N-Hydroxysuccinimide Ester; N-α-(9-Fluorenylmethoxycarbonyl)-N-ε-[(2,5-dioxo-1-pyrrolidinyl)oxy]carbonyl-L-lysine (related derivative); N-Succinimidyl Glutamate; Glutamic Acid NHS Ester; 1-[(5-Carboxypentanoyl)oxy]-2,5-pyrrolidinedione; N-Hydroxysuccinimide Ester of Glutaric Acid Monocarboxylate
EINECS	Contact for details

Quality Control

Our 5-(2,5-Dioxopyrrolidin-1-yl)oxy-5-oxopentanoic acid is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation by NMR and IR spectroscopy, to ensure it meets the high standards required for research and bioconjugation applications. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C (refrigerated) or as indicated on the label or COA. This product is hygroscopic (moisture-sensitive); ensure containers are sealed tightly after each use to minimize exposure to atmospheric moisture. For long-term storage, consider storing under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Assay	95.0% - 105.0%
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.1%
Solubility	Soluble in DMF, DMSO; slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.