Description

L-Serine 7-Amido-4-Methylcoumarin Hydrochloride is a specialized fluorogenic substrate and biochemical reagent. This compound is critical for research and development in enzymology and diagnostic assay development, serving as a highly sensitive probe for specific protease activity. It is primarily needed by biotechnology, pharmaceutical, and diagnostic manufacturing industries for high-throughput screening and biochemical analysis.

Application

• Protease Activity Assays: A key fluorogenic substrate for detecting and quantifying serine protease activity in research and diagnostic kits.
• High-Throughput Screening (HTS): Used in pharmaceutical R&D for screening libraries of compounds against specific protease targets.
• Enzyme Kinetics Studies: Enables precise measurement of kinetic parameters (Km, Vmax) for enzymatic reactions in biochemical research.
• Diagnostic Reagent Manufacturing: Incorporated as a core component in the production of clinical diagnostic assays for various disease markers.
• Cell Biology & Immunology Research: Utilized to study protease involvement in cellular processes, signaling pathways, and immune responses.
• Biomarker Detection: Supports the development of sensitive detection methods for proteolytic biomarkers associated with disease states.

Basic Information

Product Name	L-Serine 7-Amido-4-Methylcoumarin Hydrochloride
CAS No.	115918-60-0
Molecular Formula	C14H15N3O5 • HCl
Molecular Weight	357.75 g/mol
Synonyms	L-Ser-AMC HCl; 7-Amino-4-methylcoumarin-L-serinamide hydrochloride; AMC-L-Ser; Ser-AMC hydrochloride; L-Serine 7-amido-4-methylcoumarin HCl; Protease Substrate L-Ser-AMC; Fluorogenic Serine Protease Substrate; (S)-2-Amino-3-hydroxy-N-(4-methyl-2-oxo-2H-chromen-7-yl)propanamide hydrochloride
EINECS	Contact for details

Quality Control

Our L-Serine 7-Amido-4-Methylcoumarin Hydrochloride is manufactured under strict quality systems to ensure batch-to-batch consistency and high purity for sensitive research and diagnostic applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation. Certificates of Analysis (COA) detailing all specifications are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This product is hygroscopic (moisture-sensitive) and should be handled in a dry environment to prevent degradation. For long-term storage, consider desiccant.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/IR)	Conforms to reference standard
Assay (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Heavy Metals	≤ 20 ppm
Residual Solvents	Meets ICH guidelines
Fluorogenic Activity	Functional test passes

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.