Description

Pro-Phe-Arg 7-Amido-4-Methylcoumarin Hydrochloride is a high-purity, fluorogenic peptide substrate designed for sensitive enzymatic assays. This compound matters for researchers and quality control labs requiring precise, reliable detection of protease activity, particularly for enzymes like trypsin and related serine proteases. It is essential for professionals in pharmaceutical R&D, diagnostic kit manufacturing, and biochemical research who need a specific and sensitive tool for kinetic studies and inhibitor screening.

Application

• Protease Activity Assays: Primary substrate for the fluorometric determination of trypsin, thrombin, and other serine proteases in research and diagnostic applications.
• Pharmaceutical Research & Development: Used in high-throughput screening (HTS) platforms to identify and characterize novel protease inhibitors for drug discovery.
• Diagnostic Manufacturing: A key component in the formulation of clinical diagnostic kits for measuring protease levels in biological samples.
• Biochemical Research: Enables detailed kinetic analysis and mechanistic studies of enzyme-substrate interactions in academic and industrial laboratories.
• Quality Control in Bioprocessing: Monitors protease activity during the production and purification of biopharmaceuticals to ensure process consistency and product purity.

Basic Information

Product Name	Pro-Phe-Arg 7-Amido-4-Methylcoumarin Hydrochloride
CAS No.	115918-56-4
Molecular Formula	C31H39N7O5 • HCl
Molecular Weight	626.15 g/mol
Synonyms	H-Pro-Phe-Arg-AMC • HCl; Pro-Phe-Arg-7-amido-4-methylcoumarin hydrochloride; Boc-Val-Pro-Arg-AMC (Note: verify specific Boc-protection); Fluorogenic substrate for trypsin; AMC substrate Pro-Phe-Arg; Trypsin substrate III; Boc-Val-Pro-Arg-MCA (MCA is equivalent to AMC); L-Prolyl-L-phenylalanyl-L-arginine 7-amido-4-methylcoumarin hydrochloride
EINECS	Contact for details

Quality Control

Our Pro-Phe-Arg 7-Amido-4-Methylcoumarin Hydrochloride is manufactured under strict quality systems to ensure batch-to-batch consistency and high performance in sensitive assays. Each lot undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation. Certificates of Analysis (COA) detailing all specifications are available upon request to support your regulatory and quality documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation from atmospheric moisture.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/IR)	Conforms to reference standard
Assay (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.