Description

4-Nitrophenyl-o-(6-o-Benzyl)-Glucopyranosyl(1--4)-o-Glucopyranosyl(1--4)-o-Glucopyranosyl(1--4)-o-Glucopyranosyl(1--4)-Glucopyranoside is a specialized, high-purity synthetic oligosaccharide derivative, primarily used as a critical chromogenic substrate in enzymatic assays. Its value lies in providing precise, reliable results for research and diagnostic applications, enabling the quantification of specific enzyme activities. This compound is essential for biochemical research, pharmaceutical development, and the production of diagnostic kits, where consistent quality and performance are paramount.

Application

• Enzymatic Substrate for α-Amylase/Glycosidase Assays: Acts as a specific chromogenic substrate for measuring the activity of enzymes like α-amylase and related glycosidases in research and clinical diagnostics.
• Biochemical Research & Drug Discovery: Used in high-throughput screening (HTS) and mechanistic studies to investigate enzyme kinetics, inhibition, and carbohydrate metabolism pathways.
• Diagnostic Kit Manufacturing: A key raw material in the formulation of diagnostic reagents and test kits for pancreatic function, metabolic disorders, and industrial enzyme analysis.
• Carbohydrate Chemistry Studies: Serves as a model compound or intermediate in synthetic carbohydrate chemistry for studying glycosylation reactions and oligosaccharide synthesis.
• Quality Control in Enzyme Production: Employed as a standard reference material to calibrate and validate the potency and purity of enzyme preparations in biomanufacturing.

Basic Information

Product Name	4-Nitrophenyl-o-(6-o-Benzyl)-Glucopyranosyl(1--4)-o-Glucopyranosyl(1--4)-o-Glucopyranosyl(1--4)-o-Glucopyranosyl(1--4)-Glucopyranoside
CAS No.	115850-12-9
Molecular Formula	C55H73NO31
Molecular Weight	1244.15 g/mol
Synonyms	4-Nitrophenyl α-maltopentaoside 6III,6IV,6V-tri-O-benzyl ether; PNP-α-G5 (6III,6IV,6V-tri-O-benzyl); 4-Nitrophenyl α-1,4-linked glucopentaoside derivative; Chromogenic amylase substrate (benzylated); 6III,6IV,6V-Tri-O-benzyl-4-nitrophenyl-α-maltopentaoside; Benzylated PNP-α-maltopentaoside; Synthetic oligosaccharide substrate
EINECS	Contact for details

Quality Control

Every batch of our 4-Nitrophenyl oligosaccharide derivative is manufactured under controlled conditions and undergoes rigorous analytical testing to ensure high purity and batch-to-batch consistency. Our quality system is designed to meet the exacting standards required for research and diagnostic applications. A comprehensive Certificate of Analysis (COA) detailing identity, purity, and assay results is provided with each shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. For long-term stability, store desiccated under inert atmosphere if the container is opened frequently.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Assay (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Specific Rotation	Contact for details
Solubility	Soluble in DMSO, DMF; slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.