Description

n-Anthraniloyl-Alanyl-Alanyl-Phenylalanyl-4-Nitroanilide CAS NO 115664-72-7 is a specialized synthetic peptide substrate designed for high-precision enzymatic research. This compound serves as a critical tool for studying the activity and kinetics of specific proteases, particularly those involved in complex biochemical pathways. It is primarily utilized by research scientists and quality control laboratories in the pharmaceutical, biotechnology, and diagnostic reagent development sectors.

Application

• Protease Enzyme Assays: As a chromogenic substrate for the quantitative determination of protease activity and inhibition studies.
• Biochemical Research: For investigating enzyme mechanisms, substrate specificity, and kinetic parameters in academic and industrial R&D settings.
• Pharmaceutical Development: Used in high-throughput screening (HTS) platforms to identify and characterize novel therapeutic enzyme inhibitors.
• Diagnostic Reagent Manufacturing: A key component in the formulation of test kits for clinical and research laboratory diagnostics.
• Quality Control (QC) Testing: Employed as a reference standard to validate the performance and consistency of enzymatic processes in bioproduction.

Basic Information

Product Name	n-Anthraniloyl-Alanyl-Alanyl-Phenylalanyl-4-Nitroanilide
CAS No.	115664-72-7
Molecular Formula	C31H33N7O7
Molecular Weight	615.64 g/mol
Synonyms	Anthraniloyl-AAF-pNA; AAF-pNA; Abz-Ala-Ala-Phe-pNA; 2-Aminobenzoyl-Ala-Ala-Phe-4-nitroanilide; Protease Substrate IV; 4-Nitroanilide derivative of Anthraniloyl-AAF; Enzymatic Substrate 115664-72-7; N-(2-Aminobenzoyl)-L-alanyl-L-alanyl-L-phenylalanyl-4-nitroanilide
EINECS	Contact for details

Quality Control

Every batch of n-Anthraniloyl-Alanyl-Alanyl-Phenylalanyl-4-Nitroanilide is manufactured under strict quality management systems. The product undergoes comprehensive analytical testing, including HPLC for purity, to ensure it meets the exacting standards required for sensitive biochemical applications. A Certificate of Analysis (COA) detailing identity, purity, and assay results is provided with each shipment to guarantee traceability and performance.

Storage

Preserve in a tightly closed container, protected from light. Store at controlled room temperature (15-25°C) in a dry environment. Due to its hygroscopic (moisture-sensitive) nature, the container must be kept tightly sealed after each use to prevent degradation from atmospheric moisture.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Purity (HPLC)	≥ 95.0%
Assay	≥ 98.0% (on dried basis)
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in aqueous buffers

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.