Description

Pglu-Gly-Arg p-Nitroanilide Hydrochloride is a specialized chromogenic peptide substrate designed for the selective detection and quantification of specific protease activity. This compound is critical for researchers and quality control laboratories requiring precise, reliable, and reproducible enzymatic assays. It is primarily utilized in the life sciences, pharmaceutical development, and diagnostic reagent manufacturing sectors for studying enzyme kinetics and developing diagnostic tests.

Application

• Protease Activity Assays: As a chromogenic substrate for the specific detection and kinetic analysis of target proteases, including thrombin and related serine proteases.
• Diagnostic Reagent Manufacturing: A key raw material in the production of in-vitro diagnostic (IVD) kits for coagulation disorders and other clinical tests.
• Pharmaceutical Research & Development: Used in high-throughput screening (HTS) to identify and characterize potential protease inhibitors as drug candidates.
• Biochemical Research: Essential for fundamental studies on enzyme mechanisms, substrate specificity, and proteolytic pathways in academic and industrial settings.
• Quality Control in Biologics: Employed to monitor and ensure the consistency and activity of enzyme-based biopharmaceutical products during manufacturing.

Basic Information

Product Name	Pglu-Gly-Arg p-Nitroanilide Hydrochloride
CAS No.	115389-02-1
Molecular Formula	C20H28N8O7 • HCl
Molecular Weight	529.95 g/mol
Synonyms	Pyr-Gly-Arg pNA • HCl; Pyroglutamyl-Glycyl-Arginine 4-Nitroanilide Hydrochloride; H-pGlu-Gly-Arg-pNA • HCl; Chromogenic Substrate S-2366 (related); Thrombin Substrate; Tos-Gly-Pro-Arg-pNA (structural analog); Benzoyl-Phe-Val-Arg-pNA (functional analog); Protease Substrate 115389-02-1
EINECS	Contact for details

Quality Control

Our Pglu-Gly-Arg p-Nitroanilide Hydrochloride is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity, identity confirmation by IR and NMR, and verification of chromogenic performance. We provide comprehensive Certificates of Analysis (COA) with each shipment, detailing all specifications and test results to ensure compliance with your research and production standards.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C (refrigerated) or as indicated on the label or COA. This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation from atmospheric moisture.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Conforms to reference
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Residue on Ignition	≤ 0.5%
Chromogenic Activity	Conforms to reference standard
Solubility	Soluble in water, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.