Description

5'-O-(4,4'-Dimethoxytrityl)-N2-Phenoxyacetyl-2'-Deoxyguanosine is a high-purity, protected nucleoside phosphoramidite precursor, a critical building block in automated oligonucleotide synthesis. This compound is essential for ensuring high coupling efficiency and sequence fidelity during the solid-phase synthesis of DNA and RNA strands. It is primarily required by biotechnology, pharmaceutical, and diagnostic research sectors for the development of primers, probes, antisense oligonucleotides, and other therapeutic nucleic acids.

Application

• Oligonucleotide Synthesis: As a key phosphoramidite monomer for the automated solid-phase synthesis of DNA sequences.
• Therapeutic Nucleic Acid Development: Production of antisense oligonucleotides, siRNA, and aptamers for pharmaceutical research.
• Diagnostic Probe Manufacturing: Synthesis of labeled probes for PCR, qPCR, FISH, and microarray applications.
• Research & Development: A fundamental reagent in academic and industrial labs for molecular biology and genetic engineering studies.
• CRISPR Guide RNA Synthesis: Used in the production of synthetic guide RNAs (sgRNAs) for genome editing technologies.

Basic Information

Product Name	5'-O-(4,4'-Dimethoxytrityl)-N2-Phenoxyacetyl-2'-Deoxyguanosine
CAS No.	115388-95-9
Molecular Formula	C39H37N5O8
Molecular Weight	703.75 g/mol
Synonyms	DMTr-Pac-dG; N2-Phenoxyacetyl-5'-O-(4,4'-dimethoxytrityl)-2'-deoxyguanosine; 2'-Deoxyguanosine, 5'-O-[bis(4-methoxyphenyl)phenylmethyl]-N2-(phenoxyacetyl)-; Pac-dG(DMTr); dG(Pac) Phosphoramidite precursor; Protected 2'-Deoxyguanosine Derivative
EINECS	Contact for details

Quality Control

Our 5'-O-(4,4'-Dimethoxytrityl)-N2-Phenoxyacetyl-2'-Deoxyguanosine is manufactured under strict quality systems to ensure batch-to-batch consistency and high purity, essential for sensitive oligonucleotide synthesis. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and moisture content. We adhere to GMP principles for production and control, supporting its use in research and development towards therapeutic applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (e.g., argon or nitrogen) in a dry, freezer. This product is hygroscopic (moisture-sensitive) and light-sensitive; prolonged exposure to ambient conditions or moisture will compromise stability and performance. Allow the sealed container to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in acetonitrile, DCM, DMF

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.