Description

H-Asn-Amc Tfa is a specialized, high-purity fluorogenic peptide substrate designed for sensitive enzymatic assays. This compound matters for researchers and quality control labs requiring precise detection and quantification of protease activity, particularly for enzymes like legumain or asparaginyl endopeptidases. It is essential for professionals in pharmaceutical R&D, biochemical research, and diagnostic kit development who need reliable, consistent reagents for high-throughput screening and mechanistic studies.

Application

• Protease Activity Assays: Primary substrate for fluorometric detection of legumain and related cysteine proteases.
• High-Throughput Screening (HTS): Used in drug discovery platforms to identify and characterize protease inhibitors.
• Biochemical Research: Tool for studying enzyme kinetics, specificity, and catalytic mechanisms in vitro.
• Diagnostic Development: Key component in developing assays for disease biomarkers associated with protease activity.
• Cell-Based Studies: Employed in live-cell imaging to monitor intracellular protease activity.
• Quality Control: Used as a reference standard in the production and validation of enzymatic diagnostic kits.

Basic Information

Product Name	H-Asn-Amc Tfa
CAS No.	115047-90-0
Molecular Formula	C₁₇H₂₀F₃N₃O₇
Molecular Weight	435.35 g/mol
Synonyms	N-[(9H-Fluoren-9-ylmethoxy)carbonyl]-L-asparaginyl-7-amido-4-methylcoumarin Trifluoroacetate; Fmoc-Asn-AMC·TFA; L-Asparagine-7-amido-4-methylcoumarin derivative; Legumain Substrate; Asparaginyl Endopeptidase Substrate; Fluorogenic Peptide Substrate AMC; Z-Ala-Ala-Asn-AMC (related); AAN-AMC
EINECS	Contact for details

Quality Control

Every batch of H-Asn-Amc Tfa is manufactured under strict quality management systems and undergoes rigorous analytical testing to ensure identity, purity, and performance. We provide comprehensive Certificates of Analysis (COA) with each shipment, detailing specifications such as HPLC purity, absorbance characteristics, and functional activity in standard assay conditions. Our quality commitment supports applications requiring reproducible and reliable results in critical research and development.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Retention time corresponds to reference standard
Identification (MS)	Consistent with molecular weight
Purity (HPLC)	≥ 95.0%
Absorbance (UV-Vis)	Characteristic profile
Water Content (KF)	≤ 2.0%
Functional Activity	≥ 90% of reference standard in legumain assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.