Description

Fluorescein-5(6)-Carboxamidocaproic Acid n-Succinimidyl Ester is a high-purity, amine-reactive fluorescent labeling reagent widely used in life science research and diagnostics. Its core value lies in its ability to covalently attach a bright, green-fluorescent fluorescein tag to proteins, antibodies, peptides, and other biomolecules containing primary amines, enabling sensitive detection and analysis. This product is essential for researchers and manufacturers in bioconjugation, immunoassay development, flow cytometry, and fluorescence microscopy who require reliable and efficient labeling chemistry.

Application

• Antibody and Protein Labeling: Conjugation of fluorescent probes to monoclonal antibodies, enzymes, and other proteins for use in immunoassays (ELISA, Western Blot) and diagnostic kits.
• Biomolecule Tracking and Detection: Fluorescent tagging of peptides, oligonucleotides, and other bioactive molecules to study their localization, binding, and cellular uptake.
• Flow Cytometry Reagent Preparation: Synthesis of fluorescently-labeled antibodies and other detection reagents for cell surface marker analysis and intracellular staining.
• Fluorescence Microscopy and Imaging: Preparation of targeted fluorescent probes for visualizing specific structures or molecules in fixed and live cells.
• Bioconjugation and Chemical Biology: A key building block in the synthesis of more complex fluorescent probes, biosensors, and drug delivery systems.
• Diagnostic Manufacturing: Production of fluorescent components for point-of-care tests and clinical diagnostic assays requiring high sensitivity and stability.

Basic Information

Product Name	Fluorescein-5(6)-Carboxamidocaproic Acid n-Succinimidyl Ester
CAS No.	114616-31-8
Molecular Formula	C33H29N3O10
Molecular Weight	627.60 g/mol
Synonyms	5(6)-FAM, SE; 5(6)-Carboxyfluorescein N-succinimidyl ester; 6-FAM, SE; Fluorescein-5(6)-isothiocyanate isomer; FITC isomer NHS ester; 5(6)-Carboxyfluorescein succinimidyl ester; FAM, SE isomer; Fluorescein NHS ester (isomeric mixture)
EINECS	Contact for details

Quality Control

Our Fluorescein-5(6)-Carboxamidocaproic Acid n-Succinimidyl Ester is manufactured under strict quality control protocols to ensure batch-to-batch consistency and high performance in sensitive applications. Each lot is characterized by advanced analytical techniques including HPLC, NMR, and mass spectrometry to confirm identity, purity, and labeling efficiency. Comprehensive Certificates of Analysis (COA) detailing all specifications are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis. For long-term stability, store under inert atmosphere.

Specification

Item	Specification
Appearance	Orange to red powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95% (Isomeric mixture)
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMF, DMSO, acetonitrile
Functional Performance	Conforms to labeling efficiency test

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.