Description

C6-Nbd-Dihydroceramide is a fluorescently labeled derivative of dihydroceramide, a key intermediate in the sphingolipid biosynthesis pathway. This compound is specifically engineered for advanced research applications, offering a powerful tool for visualizing and tracking cellular lipid dynamics. It is primarily utilized by researchers in academic, pharmaceutical, and biotechnology laboratories focused on lipid metabolism, cell signaling, and biomarker discovery.

Application

• Biomedical Research: As a fluorescent probe for studying sphingolipid metabolism, trafficking, and intracellular localization in live or fixed cells.
• Mechanistic Studies: Investigating the role of dihydroceramide and ceramide in cellular processes such as apoptosis, autophagy, and stress response.
• Drug Discovery & Screening: Enabling high-content screening assays to identify modulators of enzymes in the sphingolipid pathway (e.g., ceramide synthases, desaturases).
• Diagnostic Development: Potential use in developing fluorescent-based assays for detecting enzymatic activity or lipid-related biomarkers.
• Lipidomics: Serving as a critical standard or tracer in mass spectrometry-based lipidomic analyses to quantify endogenous sphingolipid species.

Basic Information

Product Name	C6-Nbd-Dihydroceramide
CAS No.	114301-95-0
Molecular Formula	C35H57N3O6
Molecular Weight	615.85 g/mol
Synonyms	N-[(1E,3R)-1,3-Dihydroxy-4,5-octadecadien-1-yl]-6-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]hexanamide; NBD C6-Dihydroceramide; 6-((7-Nitrobenzo[c][1,2,5]oxadiazol-4-yl)amino)-N-((2S,3R,E)-1,3-dihydroxyoctadec-4-en-2-yl)hexanamide; Fluorescent Dihydroceramide (C6-NBD); NBD-C6-Dihydroceramide
EINECS	Contact for details

Quality Control

Our C6-Nbd-Dihydroceramide is produced under stringent quality protocols to ensure high purity and batch-to-batch consistency, suitable for sensitive research applications. Each lot is characterized by advanced analytical techniques including HPLC, NMR, and mass spectrometry. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profile is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and light-sensitive. For long-term storage, keep under an inert atmosphere. Allow the vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	Yellow to orange solid or film
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Solubility	Soluble in DMSO, ethanol, chloroform

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.