Description

P(3)-1-(2-Nitrophenyl)Ethylcytidine-5'-Triphosphate is a chemically modified nucleoside triphosphate analog, specifically engineered for advanced biochemical research and diagnostic applications. Its core value lies in its ability to act as a substrate or inhibitor for DNA/RNA polymerases, enabling precise studies in enzymology, molecular biology, and the development of novel therapeutic agents. This high-purity biochemical is essential for researchers and developers in the pharmaceutical, biotechnology, and diagnostic sectors who require reliable, well-characterized building blocks for nucleic acid synthesis and probe development.

Application

• Enzymology Research: Used as a specialized substrate to study the kinetics and mechanisms of DNA and RNA polymerases, reverse transcriptases, and other nucleic acid-processing enzymes.
• Probe Synthesis: Incorporated into oligonucleotides to create labeled probes for hybridization assays, FISH (Fluorescence In Situ Hybridization), and other detection methodologies.
• Drug Discovery: Serves as a key intermediate or tool compound in the research and development of antiviral and anticancer nucleoside analog therapeutics.
• Diagnostic Assay Development: Employed in the formulation of reagents for next-generation sequencing (NGS), PCR-based diagnostics, and other molecular diagnostic platforms.
• Bioconjugation & Labeling: The reactive 2-nitrophenyl ethyl group provides a handle for further chemical modification and conjugation to other molecules or solid supports.
• Structural Biology Studies: Used in X-ray crystallography and NMR studies to investigate enzyme-substrate interactions and catalytic mechanisms.

Basic Information

Item	Detail
Product Name	P(3)-1-(2-Nitrophenyl)Ethylcytidine-5'-Triphosphate
CAS No.	114119-93-6
Molecular Formula	C₁₈H₂₃N₄O₁₆P₃
Molecular Weight	668.33 g/mol
Synonyms	1-(2-Nitrophenyl)ethyl-cytidine-5'-triphosphate; 2'-Deoxy-NPE-caged CTP; (2-Nitrophenyl)ethyl-caged Cytidine Triphosphate; NPE-Caged CTP; Caged CTP, NPE derivative; 1-(2-Nitrophenylethyl)-Cytidine-5'-triphosphate; P3-1-(2-Nitrophenyl)ethyl-CTP
EINECS	Contact for details

Quality Control

Every batch of P(3)-1-(2-Nitrophenyl)Ethylcytidine-5'-Triphosphate is manufactured and tested under stringent quality management systems to ensure the highest standards of purity and consistency required for critical research. Our products undergo rigorous analytical testing, including HPLC, NMR, and mass spectrometry, to confirm identity, potency, and the absence of critical impurities. Certificates of Analysis (COA) detailing all specifications and test results are provided with each shipment and are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below. This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability. For long-term storage, keep desiccated.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms
Identification (NMR)	Conforms
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in water, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.