Description

Boc-Gln-Ala-Arg-7-Amino-4-Methylcoumarin is a specialized fluorogenic peptide substrate designed for high-sensitivity enzymatic assays. This compound is critical for researchers and developers requiring precise detection and quantification of protease activity, particularly trypsin and related enzymes. It is an essential tool for biochemical research, pharmaceutical development, and diagnostic applications where accurate enzyme kinetics are measured.

Application

• Protease Activity Assays: Primary substrate for the continuous fluorometric measurement of trypsin, thrombin, and other serine proteases.
• Biochemical Research: Used in enzyme kinetics studies, inhibitor screening, and mechanistic investigations of proteolytic enzymes.
• Pharmaceutical Development: Employed in high-throughput screening (HTS) platforms to identify and characterize novel protease inhibitors as drug candidates.
• Diagnostic Reagent Manufacturing: A key component in the formulation of diagnostic kits for clinical analysis of blood coagulation factors and pancreatic function.
• Cell Biology Studies: Applied in assays to detect and localize protease activity within living cells or tissue samples.

Basic Information

Item	Details
Product Name	Boc-Gln-Ala-Arg-7-Amino-4-Methylcoumarin
CAS No.	113866-20-9
Molecular Formula	C₃₀H₄₅N₉O₉
Molecular Weight	663.74 g/mol
Synonyms	Boc-Gln-Ala-Arg-AMC; Boc-Gln-Ala-Arg-7-Amido-4-methylcoumarin; t-Butyloxycarbonyl-Glutaminyl-Alanyl-Arginyl-7-Amino-4-Methylcoumarin; Boc-Q-A-R-AMC; Fluorogenic Substrate for Trypsin; Trypsin Substrate Boc-Gln-Ala-Arg-AMC; Protease Substrate 113866-20-9; t-BOC-L-Glutaminyl-L-Alanyl-L-Arginine 7-Amido-4-methylcoumarin
EINECS	Contact for details

Quality Control

Our Boc-Gln-Ala-Arg-7-Amino-4-Methylcoumarin is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity verification and identity confirmation by mass spectrometry (MS) and infrared (IR) spectroscopy. A Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment to ensure traceability and compliance with research-grade specifications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Retention time corresponds to reference standard
Identification (MS)	Consistent with molecular structure
Purity (HPLC)	≥ 95.0%
Assay	≥ 98.0% (on anhydrous basis)
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.