Description

Boc-Asp(Obzl)-Pro-Arg-Amc Hcl is a protected peptide sequence featuring a C-terminal fluorogenic amide (Aminomethylcoumarin, Amc). This compound is a specialized biochemical tool primarily used as a high-purity substrate for the study and characterization of proteolytic enzymes, particularly those with trypsin-like specificity. It is essential for researchers and developers in the fields of biochemistry, enzymology, and pharmaceutical discovery. The product is supplied as a hydrochloride salt to ensure stability and solubility for experimental use.

Application

• Enzyme Substrate for Protease Research: Acts as a sensitive fluorogenic substrate for kinetic assays and inhibition studies of serine proteases, including thrombin, trypsin, and related enzymes.
• High-Throughput Screening (HTS): Utilized in drug discovery platforms to identify and characterize novel protease inhibitors for therapeutic targets.
• Biochemical Mechanism Studies: Enables detailed investigation of enzyme specificity, catalytic efficiency, and reaction mechanisms in purified systems.
• Diagnostic Reagent Development: Serves as a key component in developing in-vitro diagnostic assays for measuring protease activity in clinical samples.
• Quality Control in Peptide Synthesis: Used as a reference standard or intermediate in the quality control of custom peptide manufacturing.

Basic Information

Product Name	Boc-Asp(Obzl)-Pro-Arg-Amc Hcl
CAS No.	113866-00-5
Molecular Formula	C₃₆H₄₈N₈O₉ • HCl
Molecular Weight	773.28 g/mol (Free base)
Synonyms	Boc-Asp(OBzl)-Pro-Arg-AMC hydrochloride; t-Butyloxycarbonyl-Aspartic acid β-benzyl ester-Proline-Arginine-7-Amino-4-methylcoumarin hydrochloride; Boc-D-Asp(OBzl)-Pro-Arg-AMC • HCl (stereochemistry dependent); Fluorogenic Peptide Substrate Boc-Asp-Pro-Arg-AMC; Thrombin Substrate Analog; Protease Substrate 113866-00-5; BOC-ASP(OBZL)-PRO-ARG-AMC HCL
EINECS	Contact for details

Quality Control

Our Boc-Asp(Obzl)-Pro-Arg-Amc Hcl is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing to ensure identity, purity, and performance as a biochemical reagent. Certificates of Analysis (COA) detailing purity by HPLC, identity by mass spectrometry (MS) and NMR, and other critical parameters are available upon request. We support research and development with materials produced to meet the exacting standards required for reproducible scientific results.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a freezer. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and moisture uptake. For long-term storage, consider under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.