Description

Boc-Val-Arg-Amc Hcl CAS NO 113865-96-6 is a protected tripeptide derivative, specifically a fluorogenic substrate, featuring a C-terminal 7-amino-4-methylcoumarin (AMC) group. This compound is critically important for the sensitive and specific detection of protease activity, particularly for enzymes like thrombin and trypsin. It is an essential research tool for biochemists and pharmaceutical scientists working in enzymology, drug discovery, and diagnostic assay development.

Application

• Protease Activity Assays: Primary substrate for the fluorometric determination of trypsin-like serine protease activity.
• Enzyme Kinetic Studies: Used to measure kinetic parameters (Km, Vmax, kcat) for target proteases in biochemical research.
• High-Throughput Screening (HTS): A key reagent in screening libraries for novel protease inhibitors in drug discovery pipelines.
• Diagnostic Development: Employed in the development of sensitive clinical diagnostic tests for conditions involving abnormal protease levels.
• Biochemical Research: Fundamental tool for studying protease specificity, function, and regulation in cellular pathways.
• Quality Control in Biologics: Used to monitor and validate the activity of protease-containing biopharmaceutical products.

Basic Information

Product Name	Boc-Val-Arg-Amc Hcl
CAS No.	113865-96-6
Molecular Formula	C27H42ClN7O6
Molecular Weight	596.12 g/mol
Synonyms	Boc-Val-Arg-AMC hydrochloride; t-Butyloxycarbonyl-Val-Arg-7-amido-4-methylcoumarin hydrochloride; Boc-Val-Arg-AMC • HCl; N-α-t-Boc-L-valyl-L-arginine 7-amido-4-methylcoumarin hydrochloride; Fluorogenic substrate Boc-Val-Arg-AMC; Thrombin substrate Boc-Val-Arg-AMC; Boc-Val-Arg-MCA • HCl (common alternate abbreviation for AMC); L-Valine, N-[(1,1-dimethylethoxy)carbonyl]-, L-arginyl-, 7-amino-4-methyl-2-oxo-2H-1-benzopyran-3-yl ester, hydrochloride
EINECS	Contact for details

Quality Control

Our Boc-Val-Arg-Amc Hcl is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive biochemical applications. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment to guarantee reproducible and reliable performance in your assays.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to warm to room temperature in a desiccator before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Purity (HPLC)	≥ 95.0%
Assay (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.