Description

3'(O)-(1-Oxyl-2,2,5,5-Tetramethyl-3-Carbonyl Pyrrolidine)-8-Azidoadenosine 5'-Triphosphate is a specialized, spin-labeled and photoactivatable ATP analog designed for advanced biochemical research. This compound is critical for studying nucleotide-binding proteins, enzyme kinetics, and dynamic structural changes using techniques like Electron Paramagnetic Resonance (EPR) spectroscopy and photoaffinity labeling. It is an essential tool for researchers in structural biology, enzymology, and drug discovery who require precise molecular probes to investigate complex biomolecular interactions and mechanisms.

Application

• EPR/Spin Labeling Studies: Acts as a paramagnetic probe for investigating the structure, dynamics, and conformational changes of ATP-binding proteins and enzymes.
• Photoaffinity Labeling: The azido group enables covalent cross-linking to target proteins upon UV irradiation, allowing for the identification and mapping of ATP-binding sites.
• Enzyme Kinetics & Mechanism: Used to study the catalytic cycles, binding affinities, and inhibition of kinases, ATPases, and other nucleotide-dependent enzymes.
• Structural Biology: Aids in determining protein structures and complexes via techniques that utilize spin labels or require site-specific tagging.
• Drug Discovery Screening: Serves as a specialized substrate or probe in high-throughput assays to identify and characterize novel enzyme inhibitors or modulators.
• Nucleic Acid Research: Can be incorporated into nucleic acids to study RNA/DNA-protein interactions or to create spin-labeled oligonucleotides.

Basic Information

Product Name	3'(O)-(1-Oxyl-2,2,5,5-Tetramethyl-3-Carbonyl Pyrrolidine)-8-Azidoadenosine 5'-Triphosphate
CAS No.	113814-58-7
Molecular Formula	C₂₀H₂₈N₁₀O₁₆P₃
Molecular Weight	829.41 g/mol
Synonyms	8-Azido-ATP, spin-labeled; 8-N₃-ATP, TEMPO-labeled; 3'-O-(2,2,5,5-Tetramethyl-1-oxyl-3-pyrrolidinylcarbonyl)-8-azidoadenosine 5'-triphosphate; 8-Azidoadenosine-5'-triphosphate, 3'-O-(1-oxyl-2,2,5,5-tetramethyl-3-pyrrolidinylcarbonyl)-; SL-ATP; Photoactivatable Spin-Labeled ATP; ATP-γ-azido, TEMPO conjugate; Proxyl-ATP
EINECS	Contact for details

Quality Control

Our 3'(O)-(1-Oxyl-2,2,5,5-Tetramethyl-3-Carbonyl Pyrrolidine)-8-Azidoadenosine 5'-Triphosphate is manufactured under stringent conditions to ensure high purity and batch-to-batch consistency, suitable for sensitive research applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity assessment and spectroscopic methods for structural confirmation. Certificates of Analysis (COA) detailing all specifications are provided with every shipment to guarantee traceability and compliance with your research requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a desiccated environment. This compound is hygroscopic (moisture-sensitive) and light-sensitive. For long-term stability, store under an inert atmosphere. Allow the vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	Off-white to light yellow solid
Identification (HPLC)	Conforms
Identification (MS)	Conforms
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in aqueous buffer, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.