Description

Z-D-Arg-Gly-Arg-Pna 2 Hcl is a synthetic peptide substrate, specifically a chromogenic p-nitroanilide (pNA) derivative, used extensively in biochemical research and diagnostic development. This compound is a critical tool for the study and quantification of serine protease activity, particularly for enzymes like thrombin and trypsin. It is essential for researchers and manufacturers in the fields of pharmaceutical R&D, clinical diagnostics, and enzymology who require high-purity, reliable substrates for kinetic assays and inhibitor screening.

Application

• Protease Activity Assays: Primary substrate for the spectrophotometric determination of thrombin, trypsin, and related serine protease activity.
• Drug Discovery Screening: Used in high-throughput screening (HTS) platforms to identify and characterize novel protease inhibitors for therapeutic development.
• Diagnostic Kit Manufacturing: A key component in the formulation of chromogenic test kits for clinical coagulation analysis and other diagnostic panels.
• Biochemical Research: Fundamental tool for studying enzyme kinetics, specificity, and catalytic mechanisms in academic and industrial laboratories.
• Quality Control in Biologics: Employed to monitor and validate the activity and purity of enzyme preparations in biopharmaceutical production.

Basic Information

Product Name	Z-D-Arg-Gly-Arg-Pna 2 Hcl
CAS No.	113711-77-6
Molecular Formula	C30H45Cl2N13O7
Molecular Weight	738.68 g/mol
Synonyms	Benzyloxycarbonyl-D-arginylglycyl-L-arginine 4-nitroanilide dihydrochloride; Z-D-Arg-Gly-Arg-pNA • 2HCl; Chromogenic Substrate for Thrombin; D-Arg-Gly-Arg-pNA derivative; Tosyl-Gly-Pro-Arg-p-nitroanilide acetate salt (related); Thrombin Substrate I (chromogenic); S-2238 (related analog); H-D-Arg-Gly-Arg-pNA • 2HCl
EINECS	Contact for details

Quality Control

Our Z-D-Arg-Gly-Arg-Pna 2 Hcl is manufactured under strict quality management systems to ensure batch-to-batch consistency and high performance in sensitive assays. Each lot undergoes comprehensive analytical testing, including HPLC for purity, mass spectrometry for identity confirmation, and functional activity testing. Certificates of Analysis (COA) detailing all specifications are provided with every shipment to support your quality assurance and regulatory compliance needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/MS)	Conforms to reference standard
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Residue on Ignition	≤ 0.5%
Functional Activity	≥ 95% of reference standard
Solubility	Soluble in DMSO, DMF; slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.