Description

8-Hydroxyquinoline-β-D-Galactopyranoside is a specialized glycosidic derivative of 8-hydroxyquinoline, designed as a critical substrate for enzymatic detection systems. This compound matters for its high specificity in biochemical assays, enabling precise and sensitive detection of β-galactosidase activity. It is primarily needed by research institutions, diagnostic kit manufacturers, and pharmaceutical companies engaged in molecular biology, cell-based reporter assays, and enzyme-linked detection workflows.

Application

• β-Galactosidase Reporter Assays: A key chromogenic or fluorogenic substrate for detecting and quantifying β-galactosidase expression in molecular biology and genetic research.
• Diagnostic Development: Used in the formulation of diagnostic kits and reagents for clinical and research laboratories to identify specific enzymatic activity.
• Cell Staining & Histochemistry: Employed in histological techniques for visualizing β-galactosidase activity in tissues and cell cultures, particularly in senescence assays (SA-β-gal).
• High-Throughput Screening (HTS): Serves as a vital component in drug discovery platforms for screening compounds that modulate gene expression via β-galactosidase reporter systems.
• Enzyme Kinetics Studies: Utilized by biochemists to study the kinetics, inhibition, and mechanism of action of β-galactosidase enzymes.
• Microbiology Research: Applied in bacterial genetics (e.g., blue-white screening) and studies of microbial enzyme function.

Basic Information

Product Name	8-Hydroxyquinoline-β-D-Galactopyranoside
CAS No.	113079-84-8
Molecular Formula	C₁₉H₂₃NO₈
Molecular Weight	393.39 g/mol
Synonyms	8-Hydroxyquinoline β-D-galactopyranoside; 8-HQ-β-Gal; 8-Hydroxyquinoline β-D-galactoside; β-Galactopyranoside, 8-hydroxyquinoline; 8-Quinolinol β-D-galactopyranoside; Galactopyranosyloxyquinoline; X-Galactoside (8-Hydroxyquinoline derivative); Substrate for β-galactosidase
EINECS	Contact for details

Quality Control

Our 8-Hydroxyquinoline-β-D-Galactopyranoside is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive research and diagnostic applications. Certificates of Analysis (COA) with detailed specifications are available upon request to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and light-sensitive; ensure the container is sealed tightly after each use to minimize exposure to atmospheric moisture and light.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water
Specific Rotation	Contact for details
Heavy Metals	< 10 ppm
Residual Solvents (GC)	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.