Description

Sulfosuccinimidyl-2-(3-Azido-2-Nitrobenzamido)Ethyl-1,3'-Dithiopropionate is a heterobifunctional crosslinking reagent designed for advanced bioconjugation. This compound is critical for researchers and manufacturers who require precise, stable linkages between biomolecules, such as proteins and peptides, and other surfaces or probes. It is primarily utilized in the development of diagnostic assays, targeted drug delivery systems, and advanced materials for life science research.

Application

• Bioconjugation and Crosslinking: For creating stable covalent bonds between proteins, antibodies, peptides, and other biomolecules with surfaces, polymers, or detection labels.
• Diagnostic Assay Development: Used in the manufacture of ELISA kits, lateral flow assays, and other immunoassays by immobilizing capture antibodies or antigens.
• ADC (Antibody-Drug Conjugate) Research: Serves as a linker candidate for attaching cytotoxic payloads to monoclonal antibodies in targeted cancer therapy development.
• Surface Functionalization: Modification of nanoparticles, microspheres, biosensor chips, and other solid supports for specific biomolecule attachment.
• Proteomics and Protein Labeling: Introduction of azide groups for subsequent "click chemistry" reactions, enabling site-specific labeling and pull-down experiments.
• Biomaterials Engineering: Crosslinking agent in the fabrication of hydrogels, scaffolds, and other polymeric materials with bioactive properties.

Basic Information

Product Name	Sulfosuccinimidyl-2-(3-Azido-2-Nitrobenzamido)Ethyl-1,3'-Dithiopropionate
CAS No.	112820-42-5
Molecular Formula	C20H21N7O10S3
Molecular Weight	615.61 g/mol
Synonyms	Sulfo-SANPAH; 3-(2-Pyridyldithio)propionyl hydrazide, N-[ε-maleimidocaproyloxy]succinimide ester; N-Succinimidyl 3-(2-pyridyldithio)propionate; SPDP; LC-SPDP; N-Succinimidyl 3-(2-pyridyldithio)propionate; NHS-PEG4-SS-Azide; Heterobifunctional Crosslinker, NHS ester and Pyridyldithiol
EINECS	Contact for details

Quality Control

Every batch of Sulfosuccinimidyl-2-(3-Azido-2-Nitrobenzamido)Ethyl-1,3'-Dithiopropionate undergoes rigorous analytical testing to ensure identity, purity, and performance consistency suitable for sensitive life science applications. Our quality system is designed to support GMP-grade manufacturing for diagnostic and therapeutic research. A comprehensive Certificate of Analysis (COA) detailing purity (HPLC), functional group activity, and impurity profiles is provided with each shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This compound is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake. For long-term stability, store under an inert atmosphere.

Specification

Item	Specification
Appearance	Off-white to light yellow solid
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in aqueous buffers
Functional Activity	Confirmed by model conjugation assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.