Description

n-Carbobenzoxyleucyl-Leucyl-Arginine-2-Naphthylamide CAS NO 112219-39-3 is a synthetic tripeptide derivative containing a chromogenic 2-naphthylamide group. This compound is a specialized biochemical reagent primarily used as a sensitive and specific substrate for the detection and study of proteolytic enzyme activity. It is essential for researchers and quality control laboratories in the pharmaceutical and life sciences sectors requiring precise enzyme characterization. Its structure makes it particularly valuable for assays targeting enzymes like trypsin, plasmin, and other arginine-specific proteases.

Application

• Protease/Enzyme Assay Substrate: A critical chromogenic substrate for the kinetic study and quantification of trypsin-like serine proteases in biochemical research.
• Pharmaceutical R&D: Used in high-throughput screening (HTS) platforms to identify and characterize novel protease inhibitors for drug discovery programs.
• Diagnostic Reagent Manufacturing: Employed in the formulation of diagnostic kits for clinical measurements of specific protease levels relevant to various disease states.
• Cell Biology & Physiology Studies: Facilitates the investigation of protease-mediated signaling pathways, cellular differentiation, and apoptosis.
• Quality Control in Biologics: Serves as a standard reagent for assessing the activity and purity of enzyme preparations used in therapeutic manufacturing.
• Food Science & Microbiology: Applied in research to study proteolytic activity in fermentation processes or in microbial identification systems.

Basic Information

Product Name	n-Carbobenzoxyleucyl-Leucyl-Arginine-2-Naphthylamide
CAS No.	112219-39-3
Molecular Formula	C33H46N8O6
Molecular Weight	650.78 g/mol
Synonyms	Z-Leu-Leu-Arg-2NA; CBZ-Leu-Leu-Arg-2-Naphthylamide; Carbobenzoxy-Leucyl-Leucyl-Arginine-2-Naphthylamide; N-CBZ-Leu-Leu-Arg-βNA; Leu-Leu-Arg-2NA, Z-protected; Chromogenic Substrate for Trypsin; Protease Substrate 112219-39-3; Boc-Leu-Leu-Arg-2NA (related protected form)
EINECS	Contact for details

Quality Control

Our n-Carbobenzoxyleucyl-Leucyl-Arginine-2-Naphthylamide is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing to ensure high purity, identity confirmation, and batch-to-batch consistency suitable for sensitive research applications. Certificates of Analysis (COA) detailing purity by HPLC, identity by spectroscopic methods (IR, MS), and other relevant parameters are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive); allow the sealed container to equilibrate to room temperature before opening to minimize condensation and moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Assay (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in methanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.