Description

Aminoallyl UTP is a chemically modified nucleotide triphosphate essential for advanced molecular biology and diagnostic applications. Its primary value lies in its reactive aminoallyl group, which enables efficient labeling with amine-reactive dyes or other probes for sensitive detection and analysis. This modified nucleotide is a critical reagent for researchers and manufacturers in next-generation sequencing (NGS), microarray fabrication, fluorescence in situ hybridization (FISH), and various nucleic acid amplification and labeling techniques.

Application

• Fluorescent Labeling of Nucleic Acids: Used to incorporate amine-reactive fluorophores into DNA or RNA via enzymatic incorporation for applications like microarray analysis and FISH.
• Next-Generation Sequencing (NGS): Serves as a key substrate for the synthesis of labeled nucleotides used in sequencing-by-synthesis platforms.
• Probe Synthesis: Essential for generating high-specificity, labeled probes for diagnostic assays and genomic research.
• cDNA Library Preparation: Facilitates the incorporation of aminoallyl groups into cDNA, allowing for subsequent coupling to dyes for expression profiling.
• Single Nucleotide Polymorphism (SNP) Detection: Used in assays requiring highly sensitive and specific labeled nucleotides.
• Bioconjugation: The reactive amine group allows for covalent attachment to surfaces, beads, or other molecules for biosensor development and assay platforms.

Basic Information

Product Name	Aminoallyl UTP
CAS No.	112131-73-4
Molecular Formula	C14H23N4O15P3
Molecular Weight	580.26 g/mol
Synonyms	5-(3-Aminoallyl)uridine-5'-triphosphate; AA-UTP; 5-(3-Aminoallyl)-UTP; Aminoallyl-UTP; 5-(3-Aminoallyl)uridine triphosphate; N6-(3-Aminoallyl)-UTP; Allylamino-UTP; 5-AA-UTP
EINECS	Contact for details

Quality Control

Our Aminoallyl UTP is manufactured under strict quality control protocols to ensure high purity and batch-to-batch consistency suitable for sensitive research and diagnostic applications. Each lot is thoroughly analyzed using advanced chromatographic and spectroscopic techniques. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and performance in enzymatic incorporation is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white powder or solid
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (Karl Fischer)	≤ 5.0%
Enzymatic Incorporation Test	Suitable for use

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.