Description

Phosphoenolthiopyruvate CAS NO 111537-26-9 is a sulfur-containing analog of phosphoenolpyruvate (PEP), a central high-energy intermediate in biological pathways. This compound is of significant interest in biochemical research and pharmaceutical development, particularly for studying enzyme mechanisms and metabolic pathways where the sulfur atom acts as a key probe or inhibitor. It is primarily utilized by research institutions, pharmaceutical R&D departments, and biotechnology companies engaged in enzymology, metabolic engineering, and the development of novel therapeutic agents.

Application

• Enzymology Research: A critical tool for studying the kinetics and mechanism of pyruvate kinase and other PEP-utilizing enzymes, where the thio-substitution alters reactivity.
• Metabolic Pathway Analysis: Used as a tracer or inhibitor to investigate glycolysis, gluconeogenesis, and the shikimate pathway in microbial and plant systems.
• Pharmaceutical Development: Serves as a precursor or intermediate in the synthesis of novel sulfur-containing drug candidates and enzyme inhibitors.
• Biochemical Assay Development: Employed in the design and validation of high-throughput screening assays for drug discovery targeting metabolic enzymes.
• Academic Research: Fundamental studies in bioorganic chemistry to understand phosphate and thiophosphate transfer reactions.

Basic Information

Product Name	Phosphoenolthiopyruvate
CAS No.	111537-26-9
Molecular Formula	C3H5O6PS
Molecular Weight	200.11 g/mol
Synonyms	Phosphoenolthiopyruvic Acid; (E)-Phosphoenol-3-thiopyruvate; PEP-thio analog; 2-(Phosphonooxy)-3-thioacrylic Acid; Thiophosphoenolpyruvate; 3-Thiophosphoenolpyruvate; PETP
EINECS	Contact for details

Quality Control

Our Phosphoenolthiopyruvate is manufactured under strict quality control protocols to ensure high purity and batch-to-batch consistency for research applications. Each lot is characterized by advanced analytical techniques including NMR, HPLC, and mass spectrometry. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles is provided with every shipment to support your regulatory and research documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a desiccated environment. This product is hygroscopic (moisture-sensitive) and light-sensitive (store away from light). For long-term stability, store under an inert atmosphere such as argon or nitrogen.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in water, buffer solutions

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.