Description

ε-n-Acetyl-Alpha(N)-Dansyl-Lysyl-Alanyl-Alanine is a synthetic, dansyl-labeled tetrapeptide derivative. This compound is a critical tool in biochemical and pharmaceutical research, primarily serving as a high-sensitivity fluorescent substrate or probe. It is essential for researchers and manufacturers in proteomics, enzymology, and diagnostic assay development who require precise, labeled peptides for studying enzyme kinetics, protein interactions, and cellular processes. The product is supplied as a high-purity material to ensure reliable and reproducible experimental results.

Application

• Protease & Enzyme Substrate: Used as a specific, fluorescently-quenched substrate for studying the activity and inhibition of proteolytic enzymes like trypsin, plasmin, and other serine proteases.
• Biochemical Assay Development: Serves as a key component in the development of high-throughput screening (HTS) assays for drug discovery targeting specific proteases.
• Peptide Mapping & Protein Sequencing: Acts as a reference standard or a derivatization agent in analytical techniques for peptide identification and structural analysis.
• Fluorescence Resonance Energy Transfer (FRET) Studies: Utilized in FRET-based constructs to investigate protein-protein interactions and conformational changes in real-time.
• Diagnostic Reagent Manufacturing: Incorporated into the production of specialized diagnostic kits for detecting and quantifying specific enzyme activities in clinical samples.
• Academic & Pharmaceutical R&D: Employed in fundamental research to elucidate metabolic pathways and validate new therapeutic targets within the protease family.

Basic Information

Product Name	ε-n-Acetyl-Alpha(N)-Dansyl-Lysyl-Alanyl-Alanine
CAS No.	111290-37-0
Molecular Formula	C30H41N7O9S
Molecular Weight	675.76 g/mol
Synonyms	Ac-Dns-KAA-OH; Nε-Acetyl-Nα-dansyl-L-lysyl-L-alanyl-L-alanine; Dansyl-Lys(Ac)-Ala-Ala; Dns-Lys(Ac)-Ala-Ala; 5-(Dimethylamino)naphthalene-1-sulfonyl-L-lysyl(N6-acetyl)-L-alanyl-L-alanine; N-Acetyl-N-dansyl-L-lysyl-L-alanyl-L-alanine; Fluorescent Peptide Substrate Ac-Dns-KAA; ε-Acetyl-α-Dansyl-Lysyl-Alanyl-Alanine
EINECS	Contact for details

Quality Control

Our ε-n-Acetyl-Alpha(N)-Dansyl-Lysyl-Alanyl-Alanine is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, mass spectrometry for identity confirmation, and NMR for structural verification. We provide a detailed Certificate of Analysis (COA) with every shipment, ensuring traceability and compliance with your research and development specifications. Custom purity grades and analytical packages are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a desiccated environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a dry atmosphere before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	Yellow to light brown powder or solid
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95.0% (Area %)
Water Content (KF)	≤ 5.0 %
Heavy Metals	≤ 20 ppm
Solubility	Soluble in DMSO, DMF; slightly soluble in methanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.