Description

S-Adenosyl-L-[Methyl-3H]Methionine is a high-specific-activity, tritium-labeled form of the universal methyl donor S-adenosylmethionine (SAMe). This radiolabeled compound is an indispensable tool for tracing and quantifying methyltransferase activity in biochemical and pharmacological research. It is primarily used by scientists in pharmaceutical R&D, academic institutions, and contract research organizations (CROs) for advanced studies in epigenetics, metabolic pathways, and drug discovery.

Application

• Biochemical Research: Used as a tracer in enzymatic assays to study the kinetics and mechanisms of methyltransferases (e.g., DNA methyltransferases, histone methyltransferases, protein methyltransferases).
• Epigenetics Studies: Critical for investigating DNA and histone methylation patterns, which are central to gene expression regulation and epigenetic inheritance.
• Metabolic Pathway Analysis: Enables the tracking of one-carbon metabolism and transmethylation pathways in cell culture and tissue samples.
• Drug Discovery & Screening: Serves as a key substrate in high-throughput screening (HTS) assays to identify and characterize novel methyltransferase inhibitors or activators for therapeutic development.
• Autoradiography & Imaging: The tritium label allows for precise localization and quantification of methylation events in tissues or on gels via autoradiographic techniques.
• In Vitro Diagnostic Development: Used in the development of sensitive diagnostic assays that measure methyltransferase activity as a biomarker for certain diseases.

Basic Information

Product Name	S-Adenosyl-L-[Methyl-3H]Methionine
CAS No.	111093-45-9
Molecular Formula	C15H23N6O5S* *Tritium ([3H]) is incorporated at the methyl group.
Molecular Weight	~398.4 g/mol (unlabeled form)
Synonyms	S-Adenosyl-L-methionine, [methyl-3H]; SAMe, [methyl-3H]; AdoMet, [methyl-3H]; (3S)-5'-[(3-Amino-3-carboxypropyl)methylsulfonio]-5'-deoxyadenosine, [methyl-3H]; L-Methionine, S-adenosyl-, [methyl-3H]; SAM, [methyl-3H]; Radioactive S-adenosylmethionine; Tritiated SAM
EINECS	Contact for details

Quality Control

Our S-Adenosyl-L-[Methyl-3H]Methionine is produced and handled under stringent quality protocols to ensure batch-to-batch consistency and reliable performance in sensitive assays. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing critical parameters such as radiochemical purity, specific activity, and chemical purity. We adhere to relevant guidelines for the handling of radioactive materials to guarantee product integrity.

Storage

Preserve in a tightly closed container, protected from light. Store frozen (-20°C or below) in the original packaging. Avoid repeated freeze-thaw cycles. This product is strictly light-sensitive and must be protected from all light exposure during handling and storage to prevent radiolysis and chemical degradation.

Specification

Item	Specification
Appearance	Clear, colorless to slightly yellow solution
Identification (HPLC/TLC)	Conforms to reference standard
Specific Activity	Contact for details (typically 60-85 Ci/mmol)
Radiochemical Purity (HPLC)	≥ 95%
Chemical Purity (HPLC)	≥ 90%
Concentration	1 mCi/mL (approx.)
Solvent	Dilute aqueous sulfuric acid or custom buffer
Sterility (if applicable)	Sterile filtered (0.2 µm)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.