Description

(3'R,5'S)-3'-Hydroxycotinine Acetate is a high-purity, stereochemically defined metabolite of cotinine, a primary nicotine derivative. This compound is of significant value as a critical reference standard and analytical intermediate in pharmaceutical and toxicological research. It is primarily required by research institutions, diagnostic laboratories, and pharmaceutical companies engaged in nicotine metabolism studies, biomarker development, and forensic analysis.

Application

• Pharmaceutical Reference Standard: Used as a certified reference material (CRM) for the quantification of nicotine metabolites in biological samples via LC-MS/MS or HPLC.
• Biomarker Research: Essential for studying human nicotine metabolism, pharmacokinetics, and for validating analytical methods in clinical and pre-clinical trials.
• Diagnostic Kit Development: Serves as a key calibrant in the manufacture of immunoassay kits for nicotine exposure testing and smoking cessation monitoring.
• Forensic Toxicology: Employed as an analytical standard in forensic labs to accurately determine nicotine intake and exposure levels.
• Metabolic Pathway Studies: A crucial intermediate for investigating specific enzymatic pathways involved in cotinine and nicotine processing.
• Chemical Synthesis: Acts as a chiral building block or precursor for the synthesis of more complex nicotine analogs and labeled compounds for tracer studies.

Basic Information

Product Name	(3'R,5'S)-3'-Hydroxycotinine Acetate
CAS No.	111034-55-0
Molecular Formula	C12H14N2O3
Molecular Weight	234.25 g/mol
Synonyms	trans-3'-Hydroxycotinine Acetate; (3R,5S)-3-Hydroxycotinine Acetate; 1-Methyl-5-(3-pyridyl)-2-pyrrolidinone-3-acetate; 3'-Hydroxycotinine 3-Acetate; Nicotine Metabolite Acetate; Cotinine Hydroxy Metabolite Acetate; (R,S)-3'-Hydroxycotinine Acetate (stereoisomer mixture); 5-(3-Pyridyl)-1-methyl-2-pyrrolidinone-3-yl acetate
EINECS	Contact for details

Quality Control

Our (3'R,5'S)-3'-Hydroxycotinine Acetate is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including chiral purity verification by HPLC, to ensure identity, potency, and freedom from critical impurities. Certificates of Analysis (COA) detailing lot-specific results for assay, chiral purity, and related substances are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at controlled room temperature (15-25°C) in a dry environment. For long-term storage, consider storing under inert atmosphere at -20°C.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Assay (HPLC)	≥98.0%
Chiral Purity (Chiral HPLC)	≥99.0% (3'R,5'S isomer)
Related Substances (HPLC)	Total impurities ≤2.0%
Any single unknown impurity	≤0.5%
Residual Solvents (GC)	Complies with ICH guidelines
Water Content (KF)	≤0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.