Description

2’-o-Methyl-Ra(n-Bz)Phosphoramidite is a high-purity, protected phosphoramidite monomer essential for the solid-phase synthesis of oligonucleotides. Its primary value lies in enabling the precise and efficient incorporation of modified nucleosides, which is critical for developing advanced therapeutic and diagnostic nucleic acid analogs. This compound is a key building block for researchers and manufacturers in the pharmaceutical, biotechnology, and diagnostic industries, particularly those focused on antisense oligonucleotides, siRNA, and aptamers.

Application

• Oligonucleotide Synthesis: As a key phosphoramidite monomer for the automated solid-phase synthesis of RNA and modified DNA strands.
• Therapeutic Nucleic Acid Development: Critical for producing antisense oligonucleotides (ASOs) and small interfering RNA (siRNA) candidates for drug discovery.
• Diagnostic Probe Manufacture: Used in the synthesis of labeled probes and primers for molecular diagnostics and research assays.
• Aptamer Production: Enables the creation of chemically modified aptamers with enhanced stability and binding affinity.
• Research & Development: Serves as a vital reagent in academic and industrial R&D for studying nucleic acid structure, function, and therapeutic potential.
• Scale-Up cGMP Manufacturing: Suitable for use in current Good Manufacturing Practice (cGMP) environments for the production of clinical-grade oligonucleotide APIs.

Basic Information

Product Name	2’-o-Methyl-Ra(n-Bz)Phosphoramidite
CAS No.	110782-31-5
Molecular Formula	C41H49N4O7P
Molecular Weight	740.83 g/mol
Synonyms	2'-O-Methyladenosine, 5'-[(2-cyanoethyl)-N,N-diisopropylphosphoramidite], 3'-Bz; 2'-O-Methyl-A(Bz) Phosphoramidite; 5'-Dimethoxytrityl-2'-O-methyl-N6-benzoyladenosine-3'-[2-cyanoethyl-N,N-diisopropylphosphoramidite]; 2'-O-Me-A(Bz) CE Phosphoramidite; dA(2'-OMe) Phosphoramidite (Bz protected); 2'-O-Methyl-5'-DMT-N6-Benzoyladenosine-3'-phosphoramidite
EINECS	Contact for details

Quality Control

Every batch of 2’-o-Methyl-Ra(n-Bz)Phosphoramidite is manufactured and tested under strict quality systems to ensure it meets the high-purity standards required for oligonucleotide synthesis. Our products undergo rigorous quality testing, including HPLC, NMR, and MS analysis, to ensure compliance with industry standards. Certificates of Analysis (COA) detailing purity, identity, and moisture content are available upon request. We support development through to commercial scale with consistent quality.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a dry, moisture-free environment. This compound is hygroscopic (moisture-sensitive) and easily oxidized; prolonged exposure to air, light, or ambient humidity will compromise stability and coupling efficiency. Allow sealed vials to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Moisture Content (KF)	≤ 0.5%
Solubility	Soluble in anhydrous acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.