Description

2'-Deoxy-3'-o-(4-Benzoylbenzoyl)-1,N(6)-Ethenoadenonosine 5'-Diphosphate is a chemically modified nucleotide analog, specifically engineered for advanced biochemical research and development. This compound is a critical tool for studying enzyme kinetics, nucleotide-protein interactions, and signal transduction pathways, particularly those involving ATP-dependent processes. It is primarily utilized by research institutions, pharmaceutical R&D departments, and biotechnology companies focused on molecular biology, enzymology, and drug discovery.

Application

• Enzyme Inhibition Studies: Used as a substrate analog to investigate the mechanism and kinetics of kinases, polymerases, and other ATP-utilizing enzymes.
• Photocrosslinking Agent: The benzophenone moiety (4-Benzoylbenzoyl) enables UV-induced crosslinking, making it invaluable for mapping protein-nucleotide binding sites and identifying interacting partners.
• Biochemical Probe Development: Serves as a key intermediate in synthesizing fluorescent or affinity-labeled probes for real-time monitoring of enzymatic activity.
• Structural Biology Research: Employed in X-ray crystallography and NMR studies to elucidate the three-dimensional structure of nucleotide-binding protein complexes.
• Signal Transduction Pathway Analysis: Acts as a tool to dissect and modulate ATP-mediated signaling cascades in cellular systems.
• High-Throughput Screening (HTS): Utilized in assay development for screening compound libraries against specific nucleotide-binding targets in drug discovery campaigns.

Basic Information

Product Name	2'-Deoxy-3'-o-(4-Benzoylbenzoyl)-1,N(6)-Ethenoadenonosine 5'-Diphosphate
CAS No.	110682-79-6
Molecular Formula	C₃₀H₂₈N₅O₁₃P₂
Molecular Weight	728.52 g/mol
Synonyms	1,N6-Etheno-2'-deoxy-3'-O-[4-(benzoyl)benzoyl]adenosine 5'-diphosphate; 3'-O-(4-Benzoylbenzoyl)-2'-deoxy-1,N6-ethenoadenosine 5'-diphosphate; Bz2-εdADP; ε-ADP(Bz)₂; Photoreactive ADP analog; Benzophenone-ADP analog; Ethenoadenosine diphosphate derivative
EINECS	Contact for details

Quality Control

Our 2'-Deoxy-3'-o-(4-Benzoylbenzoyl)-1,N(6)-Ethenoadenonosine 5'-Diphosphate is produced under stringent quality management protocols to ensure batch-to-batch consistency and high purity, essential for sensitive research applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles. We adhere to ISO 9001 standards, and our quality systems support the needs of GMP-compliant research environments.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a desiccated environment. This compound is hygroscopic (moisture-sensitive) and light-sensitive. For long-term stability, store under an inert atmosphere. Allow the sealed container to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMSO, DMF; Slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.