Description

5'-O-(4,4'-Dimethoxytrityl)-N6-Phenoxyacetyl-2'-Deoxyadenosine-3'-(2-Cyanoethyl-N,N-Diisopropyl)Phosphoramidite is a high-purity, protected phosphoramidite monomer essential for the automated solid-phase synthesis of oligonucleotides. This compound is critical for ensuring high coupling efficiency and yield in the production of DNA sequences, particularly for demanding applications like antisense therapeutics and genetic diagnostics. It is a fundamental building block for biotechnology, pharmaceutical R&D, and diagnostic kit manufacturers requiring precise and reliable oligonucleotide synthesis.

Application

• Oligonucleotide Synthesis: Primary building block for the automated, solid-phase synthesis of DNA strands in research and commercial settings.
• Antisense & siRNA Therapeutics: Used in the manufacture of therapeutic oligonucleotides designed to target specific RNA sequences.
• PCR Primers & Probes: Essential for synthesizing high-fidelity primers and fluorescent probes used in polymerase chain reaction (PCR) and qPCR diagnostics.
• Gene Synthesis & Cloning: Key reagent in the construction of synthetic genes and DNA fragments for molecular biology and genetic engineering.
• Diagnostic Assay Development: Used to produce capture probes and other DNA components for in vitro diagnostic (IVD) devices and kits.
• Next-Generation Sequencing (NGS): Employed in the synthesis of oligonucleotide libraries and adapters for genomic sequencing platforms.
• Academic & Industrial Research: A standard reagent in laboratories conducting fundamental research in genetics, biochemistry, and molecular pharmacology.

Basic Information

Product Name	5'-O-(4,4'-Dimethoxytrityl)-N6-Phenoxyacetyl-2'-Deoxyadenosine-3'-(2-Cyanoethyl-N,N-Diisopropyl)Phosphoramidite
CAS No.	110543-74-3
Molecular Formula	C53H58N7O8P
Molecular Weight	928.05 g/mol
Synonyms	dA(Pac) Phosphoramidite; N6-Pac-dA Phosphoramidite; 5'-DMT-2'-dA(N6-Pac)-3'-CEP; 2'-Deoxyadenosine, 5'-O-[bis(4-methoxyphenyl)phenylmethyl]-N-[2-(phenoxyacetyl)amino]-6-(phenoxyacetamido)-, 3'-[2-cyanoethyl bis(1-methylethyl)phosphoramidite]; Deoxyadenosine Phosphoramidite (Pac protected); dA Phosphoramidite, Pac protected; Adenine Phosphoramidite Derivative
EINECS	Contact for details

Quality Control

Our 5'-O-(4,4'-Dimethoxytrityl)-N6-Phenoxyacetyl-2'-Deoxyadenosine-3'-(2-Cyanoethyl-N,N-Diisopropyl)Phosphoramidite is manufactured under strict quality systems to ensure batch-to-batch consistency for critical synthetic applications. Each lot undergoes comprehensive analytical testing, including HPLC purity and 31P NMR analysis, to confirm identity and meet stringent specifications for phosphoramidite monomers. Certificates of Analysis (COA) with detailed chromatographic data are provided to support your quality assurance and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a dry, moisture-free environment. This compound is hygroscopic (moisture-sensitive) and easily oxidized; prolonged exposure to air, light, or ambient humidity will lead to decomposition and reduced coupling efficiency. Allow the sealed vial to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Conforms to reference standard
Identification (31P NMR)	Conforms to reference standard
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.