Description

Succinimidyl-5-(Bromoacetamido)Pentanoate is a heterobifunctional crosslinking reagent featuring an amine-reactive NHS ester and a thiol-reactive bromoacetamide group. This compound is critical for creating stable, covalent linkages between biomolecules, enabling advanced bioconjugation strategies in research and development. It is primarily utilized by scientists and manufacturers in the pharmaceutical, biotechnology, and diagnostic sectors for antibody-drug conjugate (ADC) development, protein labeling, and surface immobilization.

Application

• Antibody-Drug Conjugate (ADC) Linker: A key intermediate for constructing stable, cleavable, or non-cleavable linkers connecting cytotoxic payloads to monoclonal antibodies.
• Protein and Peptide Modification: For site-specific conjugation of fluorophores, biotin, peptides, or other molecules to cysteine residues via the bromoacetyl moiety.
• Surface Functionalization: Immobilization of proteins, antibodies, or other ligands onto solid supports (e.g., biosensor chips, beads, microplates) for assay development.
• Biomaterial Engineering: Crosslinking of hydrogels or polymer matrices to incorporate bioactive peptides or proteins for tissue engineering applications.
• Diagnostic Reagent Synthesis: Preparation of enzyme-labeled antibodies or other detection conjugates for immunoassays (ELISA, lateral flow tests).
• Chemical Biology Probes: Tool compound for studying protein-protein interactions or cellular pathways through targeted crosslinking or labeling.

Basic Information

Product Name	Succinimidyl-5-(Bromoacetamido)Pentanoate
CAS No.	109880-15-1
Molecular Formula	C11H15BrN2O5
Molecular Weight	335.16 g/mol
Synonyms	5-((2-Bromoacetamido)pentanoic acid N-hydroxysuccinimide ester; SBAP; NHS-PEG4-Bromoacetamide; Bromoacetyl-PEG4-NHS ester; N-Succinimidyl 5-(bromoacetamido)pentanoate; 5-((2-Bromoacetamido)pentanoic Acid Succinimidyl Ester; (2,5-Dioxopyrrolidin-1-yl) 5-[(2-bromoacetyl)amino]pentanoate
EINECS	Contact for details

Quality Control

Our Succinimidyl-5-(Bromoacetamido)Pentanoate is manufactured under strict quality systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation (NMR, IR, MS), to ensure it meets the high standards required for sensitive bioconjugation work. A detailed Certificate of Analysis (COA) is provided with every shipment, documenting purity, assay, and critical impurity profiles.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (HPLC)	Conforms
Identification (NMR)	Conforms
Purity (HPLC)	≥ 95%
Assay	≥ 90%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMF, DMSO, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.