Description

Boc-Gly-Lys-Arg-Mca CAS NO 109358-48-7 is a specialized fluorogenic peptide substrate designed for the sensitive detection and quantification of protease activity. This compound is critical for researchers and developers in life sciences and pharmaceutical discovery, providing a reliable tool for enzyme kinetic studies and high-throughput screening assays. It is primarily utilized by biotech companies, academic research institutions, and pharmaceutical R&D departments focused on enzymology, drug discovery, and diagnostic development.

Application

• Protease Activity Assays: A key fluorogenic substrate for measuring the activity of trypsin, thrombin, and related serine proteases in biochemical research.
• High-Throughput Screening (HTS): Enables rapid screening of large compound libraries for protease inhibitors in drug discovery pipelines.
• Enzyme Kinetic Studies: Used to determine kinetic parameters (Km, Vmax, kcat) for detailed characterization of protease enzymes.
• Diagnostic Development: Serves as a core component in the development of sensitive diagnostic tests for conditions involving abnormal protease levels.
• Cell Biology Research: Applied in studies of proteolytic signaling pathways and cellular processes involving specific protease activation.
• Quality Control in Biomanufacturing: Used to monitor and validate the activity of protease-containing products or to ensure their absence in final formulations.

Basic Information

Item	Details
Product Name	Boc-Gly-Lys-Arg-Mca
CAS No.	109358-48-7
Molecular Formula	C₃₃H₅₃N₁₁O₉
Molecular Weight	747.85 g/mol
Synonyms	Boc-Gly-Lys-Arg-AMC; t-Butyloxycarbonyl-Glycyl-Lysyl-Arginyl-7-Amino-4-Methylcoumarin; Boc-Gly-Lys-Arg-7-Amido-4-Methylcoumarin; Fluorogenic Substrate for Trypsin; Boc-GKR-AMC; Boc-Gly-Lys-Arg-MCA; t-Boc-Gly-Lys-Arg-AMC; Protease Substrate 109358-48-7
EINECS	Contact for details

Quality Control

Our Boc-Gly-Lys-Arg-Mca is manufactured under strict quality systems to ensure batch-to-batch consistency and high performance in sensitive assays. Each lot is characterized by advanced analytical techniques including HPLC and mass spectrometry to confirm identity and purity. Certificates of Analysis (COA) detailing purity, assay results, and impurity profiles are provided with every shipment to support your research and compliance needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Assay	≥ 98% (on dry basis)
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.