Description

Boc-Leu-Lys-Arg-Amc Hydrochloride Salt is a high-purity, fluorogenic peptide substrate designed for sensitive enzymatic assays. This compound is critical for researchers and quality control laboratories requiring precise measurement of protease activity, particularly for enzymes like furin and related proprotein convertases. It is primarily utilized in pharmaceutical R&D, biochemical research, and diagnostic kit development for studying proteolytic pathways and screening enzyme inhibitors.

Application

• Protease Activity Assays: As a fluorogenic substrate for continuous, real-time monitoring of enzymatic activity, especially for furin and other proprotein convertases.
• Pharmaceutical Research & Development: Used in high-throughput screening (HTS) campaigns to identify and characterize novel protease inhibitors for therapeutic development.
• Biochemical Mechanism Studies: Employed in kinetic studies to determine enzyme parameters (Km, Vmax, kcat) and elucidate proteolytic mechanisms in vitro.
• Diagnostic Kit Manufacturing: Serves as a key component in the formulation of diagnostic assays for detecting and quantifying specific protease levels in clinical samples.
• Cell Biology & Virology Research: Applied in studies of viral entry and pathogenesis, where host cell proteases like furin cleave viral envelope proteins.
• Quality Control in Biopharma: Used as a reference standard or assay reagent for QC testing of biopharmaceutical products where protease activity is a critical quality attribute.

Basic Information

Product Name	Boc-Leu-Lys-Arg-Amc Hydrochloride Salt
CAS No.	109358-47-6
Molecular Formula	C31H58N10O7 • xHCl
Molecular Weight	Contact for details
Synonyms	Boc-Leu-Lys-Arg-AMC HCl; t-Butyloxycarbonyl-Leucyl-Lysyl-Arginyl-7-Amino-4-Methylcoumarin Hydrochloride; Furin Substrate Boc-Leu-Lys-Arg-AMC; Boc-L-Lys-L-Arg-AMC Hydrochloride; Fluorogenic Peptide Substrate 109358-47-6; PC Substrate; Boc-Leu-Lys-Arg-AMC • HCl; Proprotein Convertase Substrate
EINECS	Contact for details

Quality Control

Our Boc-Leu-Lys-Arg-Amc Hydrochloride Salt is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for research and diagnostic applications. Certificates of Analysis (COA) with detailed chromatographic data are provided to guarantee traceability, consistency, and reliability for your critical experiments.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This material is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in water
Fluorogenic Activity	Functional activity confirmed vs. reference standard

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.