Description

n-Acetylglucosamine-Pyrophosphorylundecaprenol is a critical lipid-linked intermediate in the biosynthesis of bacterial cell wall peptidoglycan. This compound matters as a key substrate for the MurG transferase enzyme, making it an essential biochemical tool for research into bacterial cell wall assembly and antibiotic discovery. It is primarily needed by research institutions and pharmaceutical companies engaged in microbiology, enzymology, and the development of novel antibacterial agents targeting early-stage peptidoglycan synthesis.

Application

• Biochemical Research: As a high-purity substrate for in vitro studies of the MurG glycosyltransferase enzyme and related bacterial membrane-bound biosynthesis pathways.
• Antibiotic Mechanism of Action Studies: Used to investigate and screen for inhibitors that target the lipid cycle II of peptidoglycan assembly, a validated target for new antibiotics.
• Enzyme Kinetics and Assay Development: Serves as a critical reagent for characterizing enzyme activity, specificity, and for developing high-throughput screening assays.
• Synthetic Biology: A key intermediate for reconstructing and studying minimal cell wall synthesis systems in artificial membranes or protocells.
• Glycobiology Research: Useful for studying the biosynthesis and function of complex bacterial cell surface glycopolymers.

Basic Information

Product Name	n-Acetylglucosamine-Pyrophosphorylundecaprenol
CAS No.	109138-08-1
Molecular Formula	C79H134N2O19P2
Molecular Weight	1501.86 g/mol
Synonyms	Lipid II precursor (GlcNAc); Undecaprenyl-diphospho-N-acetylglucosamine; UDP-GlcNAc:undecaprenyl-phosphate GlcNAc-1-phosphate transferase product; N-Acetylglucosaminylpyrophosphorylundecaprenol; GlcNAc-PP-Undecaprenol; (GlcNAc)1-lipid I; Bacillosamine-PP-undecaprenol (related); EINECS for this specific lipid intermediate is not commonly listed.

Quality Control

Our n-Acetylglucosamine-Pyrophosphorylundecaprenol is produced and handled under stringent conditions to ensure batch-to-batch consistency and high biochemical activity. Each lot undergoes comprehensive analytical characterization, including HPLC purity analysis, mass spectrometry (MS) for identity confirmation, and functional activity testing in enzymatic assays. Certificates of Analysis (COA) detailing all specifications are available upon request to support your critical research and development work.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere. This product is hygroscopic (moisture-sensitive) and light-sensitive. For long-term stability, store desiccated. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid or film
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 95% (Area %)
Assay (Enzymatic)	≥ 90% (Functional activity)
Solubility	Soluble in chloroform/methanol/water mixtures, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.