Description

4-Nitrophenyl 4,6-Benzylidene-A-D-Maltoheptaoside is a specialized chromogenic substrate designed for the precise detection and quantification of α-amylase activity. This compound matters because it offers a highly sensitive and specific mechanism for enzymatic analysis, which is critical for ensuring product quality and process control. It is primarily needed by researchers and quality control laboratories in the pharmaceutical, diagnostic, and food science industries for enzyme assay development and biochemical research.

Application

• Primary Substrate for α-Amylase Assays: Used as the key reagent in colorimetric and kinetic assays to measure α-amylase activity in various samples.
• Clinical Diagnostics: Employed in the development of diagnostic kits for measuring pancreatic and salivary α-amylase levels, which are biomarkers for conditions like pancreatitis.
• Pharmaceutical Quality Control: Essential for testing the enzymatic activity of amylase-containing pharmaceutical preparations and raw materials.
• Food & Beverage Industry Analysis: Applied to monitor amylase activity in processes such as brewing, baking, and starch processing to ensure consistency and quality.
• Biochemical Research: Serves as a critical tool in enzymology studies for investigating enzyme kinetics, inhibition, and the mechanism of action of α-amylase.
• Industrial Enzyme Production: Used in the fermentation and downstream processing sectors to assay and standardize commercial α-amylase preparations.

Basic Information

Product Name	4-Nitrophenyl 4,6-Benzylidene-A-D-Maltoheptaoside
CAS No.	109055-07-4
Molecular Formula	C55H75NO37
Molecular Weight	1350.18 g/mol
Synonyms	PNP-G7; 4-Nitrophenyl α-maltoheptaoside 4,6-benzylidene acetal; 4-Nitrophenyl 4,6-O-benzylidene-α-D-maltoheptaoside; Bz-α-G7-PNP; Benzylidene-PNP-α-maltoheptaoside; Chromogenic amylase substrate; α-Amylase substrate (benzylidene blocked); 4-Nitrophenyl-4,6-O-benzylidene-α-maltoheptaoside
EINECS	Contact for details

Quality Control

Our 4-Nitrophenyl 4,6-Benzylidene-A-D-Maltoheptaoside is produced and tested to meet the exacting standards required for critical research and diagnostic applications. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure high lot-to-lot consistency and reliable performance in enzymatic assays. Certificates of Analysis (COA) detailing purity, assay, and specific performance data are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). Due to its hygroscopic (moisture-sensitive) nature, the container must be kept sealed in a desiccated environment to maintain stability and prevent degradation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Assay (Enzymatic)	Suitable for α-amylase detection
Water Content (KF)	≤ 5.0%
Solubility	Soluble in water and buffer solutions

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.