Description

n-Succinyl-Ala-Ala-Val-Ala p-Nitroanilide CAS NO 108322-03-8 is a synthetic, chromogenic peptide substrate specifically designed for the detection and quantification of elastase activity. This compound is a critical reagent in biochemical research and pharmaceutical development, offering high sensitivity and specificity for enzymatic assays. It is primarily utilized by research institutions, diagnostic kit manufacturers, and pharmaceutical companies involved in studying inflammatory processes, protease function, and drug discovery.

Application

• Elastase Activity Assays: Primary substrate for the colorimetric or spectrophotometric determination of human leukocyte elastase (HLE), pancreatic elastase, and related serine proteases.
• Inflammation Research: Used in studies of inflammatory diseases such as emphysema, cystic fibrosis, and rheumatoid arthritis where elastase activity is a key biomarker.
• Drug Discovery & Screening: Essential tool in high-throughput screening (HTS) platforms to identify and characterize novel elastase inhibitors as potential therapeutic agents.
• Diagnostic Development: A key component in the formulation of diagnostic kits for measuring elastase levels in clinical and research samples.
• Enzyme Kinetics Studies: Employed to determine kinetic parameters (Km, Vmax) and inhibition constants (Ki) for elastase enzymes.
• Biochemical Research: Used in general protease research to understand substrate specificity and catalytic mechanisms.

Basic Information

Product Name	n-Succinyl-Ala-Ala-Val-Ala p-Nitroanilide
CAS No.	108322-03-8
Molecular Formula	C24H34N6O9
Molecular Weight	550.57 g/mol
Synonyms	Suc-A-A-V-A-pNA; Succinyl-Ala-Ala-Val-Ala-p-nitroanilide; N-Succinyl-Ala-Ala-Val-Ala 4-nitroanilide; Elastase Substrate IV; Suc-AAVA-pNA; Leukocyte Elastase Substrate; HLE Substrate; Peptide Substrate for Elastase; 4-Nitroanilide of Suc-A-A-V-A
EINECS	Contact for details

Quality Control

Our n-Succinyl-Ala-Ala-Val-Ala p-Nitroanilide is manufactured under strict quality systems to ensure batch-to-batch consistency and reliability for sensitive research applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity verification and functional activity testing in elastase assays. A detailed Certificate of Analysis (COA) providing purity, identity, and assay data is supplied with every shipment to support your quality assurance and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Specific Optical Rotation	Contact for details
Solubility	Soluble in DMSO, DMF; slightly soluble in water
Functional Activity	Conforms in standard elastase assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.