Description

D-Phe-Val p-Nitroanilide CAS NO 108321-89-7 is a specialized chromogenic peptide substrate designed for high-precision enzymatic assays. This compound is critical for researchers and quality control laboratories requiring accurate and reproducible measurement of protease activity. It is primarily utilized in the pharmaceutical and biotechnology sectors for enzyme kinetics studies, inhibitor screening, and diagnostic reagent development.

Application

• Protease Activity Assays: A key substrate for the quantitative determination of specific protease enzyme activities in research and development.
• Pharmaceutical Screening: Used in high-throughput screening (HTS) platforms to identify and characterize novel protease inhibitors for drug discovery.
• Diagnostic Reagent Formulation: An essential component in the development of clinical diagnostic kits for detecting enzymatic markers of disease.
• Biochemical Research: Employed in fundamental enzymology studies to investigate substrate specificity, kinetic parameters, and catalytic mechanisms.
• Quality Control (QC) Testing: Used in biopharmaceutical manufacturing QC to monitor the activity and consistency of enzyme-based products or processes.

Basic Information

Product Name	D-Phe-Val p-Nitroanilide
CAS No.	108321-89-7
Molecular Formula	C20H24N4O5
Molecular Weight	400.43 g/mol
Synonyms	D-Phenylalanyl-L-valine 4-nitroanilide; D-Phe-Val-pNA; N-(4-Nitrophenyl)-D-phenylalanyl-L-valinamide; H-D-Phe-Val-pNA; p-Nitroanilide of D-Phe-Val; Chromogenic Substrate D-Phe-Val-pNA; Protease Substrate 108321-89-7
EINECS	Contact for details

Quality Control

Our D-Phe-Val p-Nitroanilide is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the stringent requirements for research and diagnostic applications. Certificates of Analysis (COA) detailing purity, assay, and related substances are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.