Description

Trizma Citrate Dibasic CAS NO 108321-34-2 is a high-purity, dual-buffering agent combining the properties of tris(hydroxymethyl)aminomethane (Tris) and citric acid. This compound is essential for creating and maintaining precise, stable pH environments in complex biochemical and diagnostic systems. It is primarily needed by manufacturers in the pharmaceutical, biotechnology, and in-vitro diagnostic (IVD) industries for formulating buffers, reagents, and cell culture media.

Application

• Biochemical & Diagnostic Reagent Formulation: A key component in buffer systems for enzyme assays, immunoassays, and molecular biology kits.
• Pharmaceutical Buffer: Used in the preparation of parenteral solutions and ophthalmic products requiring precise pH control and biocompatibility.
• Cell Culture Media: Serves as a buffering agent in mammalian cell culture to maintain physiological pH under CO2 atmosphere.
• Chromatography: Employed in the mobile phase for ion-exchange and affinity chromatography of proteins and nucleic acids.
• Protein Crystallization: Provides a stable pH condition for screening and optimizing protein crystal growth.
• Electrophoresis Buffers: Used in capillary electrophoresis (CE) and gel electrophoresis applications.

Basic Information

Product Name	Trizma Citrate Dibasic
CAS No.	108321-34-2
Molecular Formula	C9H17NO8
Molecular Weight	267.24 g/mol
Synonyms	Tris Citrate Dibasic; 2-Amino-2-(hydroxymethyl)-1,3-propanediol Citrate (2:1); Trometamol Citrate; Trisodium Citrate Tromethamine; Trizma® Citrate; Tris-Citrate Buffer Salt; Citric Acid Tris Salt; THAM Citrate
EINECS	Contact for details

Quality Control

Our Trizma Citrate Dibasic is manufactured under a quality management system and undergoes rigorous analytical testing to ensure it meets stringent specifications for purity and consistency. We provide comprehensive Certificates of Analysis (COA) with each batch, detailing key parameters such as assay, pH, and impurity profiles. The product is suitable for applications requiring high-grade materials, including pharmaceutical and diagnostic manufacturing.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry, and well-ventilated area at a controlled room temperature (15-25°C). Due to its hygroscopic (moisture-sensitive) nature, the container must be kept tightly sealed after each use to prevent absorption of atmospheric moisture.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms
Assay (Titration)	≥ 98.0 %
pH (1% Solution)	5.5 - 6.5
Loss on Drying	≤ 2.0 %
Heavy Metals (as Pb)	≤ 10 ppm
Residue on Ignition	≤ 0.1 %
Endotoxin (where applicable)	< 0.25 EU/mg

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.