Description

Benzyloxycarbonyltyrosyl-Lysyl-Arginine-4-Nitroanilide is a specialized synthetic peptide substrate designed for enzymatic research and diagnostic applications. This compound is critical for studying the activity and kinetics of specific proteolytic enzymes, particularly serine proteases like trypsin and thrombin. It is primarily utilized by research institutions, pharmaceutical R&D departments, and diagnostic kit manufacturers who require high-purity, well-characterized biochemical tools for accurate and reproducible results.

Application

• Enzyme Kinetics Research: As a chromogenic substrate for the precise measurement of protease activity, inhibition, and kinetic parameters.
• Diagnostic Assay Development: A key component in the formulation of in-vitro diagnostic (IVD) kits for clinical testing of coagulation factors and other enzymatic pathways.
• Pharmaceutical Screening: Used in high-throughput screening (HTS) platforms to identify and characterize novel protease inhibitors for drug discovery.
• Biochemical Reagent: Serves as a standard or control reagent in quality control laboratories for enzyme-linked assays.
• Academic Research: Fundamental tool in university and institutional labs for studying proteolysis mechanisms and signal transduction pathways.

Basic Information

Product Name	Benzyloxycarbonyltyrosyl-Lysyl-Arginine-4-Nitroanilide
CAS No.	108318-36-1
Molecular Formula	C38H49N9O10
Molecular Weight	791.86 g/mol
Synonyms	Z-Tyr-Lys-Arg-pNA; Z-Tyr-Lys-Arg-4-Nitroanilide; Benzyloxycarbonyl-L-tyrosyl-L-lysyl-L-arginine 4-nitroanilide; Tosyl-Gly-Pro-Arg-pNA (common related substrate class); Chromogenic Substrate for Trypsin; Chromozym TRY; Protease Substrate 108318-36-1; Nα-Benzyloxycarbonyl-L-tyrosyl-N6-(tert-butoxycarbonyl)-L-lysyl-N5-[(benzyloxycarbonyl)aminoiminomethyl]-L-ornithine 4-nitroanilide (related protected form)
EINECS	Contact for details

Quality Control

Every batch of our peptide substrates is produced and tested under strict quality management protocols to ensure identity, purity, and performance consistency. We provide comprehensive Certificates of Analysis (COA) with each shipment, detailing key parameters such as assay purity, optical purity (HPLC), water content, and residual solvents. Our quality systems support compliance with relevant standards for research and diagnostic use.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below for long-term stability. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (Mass Spectrometry)	Conforms to structure
Assay (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in water
Extinction Coefficient (ε)	~10,000 M-1cm-1 at 405 nm (typical, in assay buffer)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.