Description

Nicotinamide Adenine Dinucleotide, [Adenine-2,8-3H] is a high-specific-activity, tritium-labeled form of the essential coenzyme NAD+. This radiolabeled compound is a critical tool for researchers, enabling the precise tracking and quantification of NAD+ in complex biochemical pathways. It is indispensable for advanced studies in metabolism, enzymology, and signal transduction within pharmaceutical R&D, academic biochemistry, and life science research sectors.

Application

• Biochemical Research & Enzyme Kinetics: Used as a tracer in studies of dehydrogenase and reductase enzyme mechanisms, substrate specificity, and reaction rates.
• Metabolic Pathway Analysis: Essential for tracing NAD+ consumption and regeneration in central metabolic pathways such as glycolysis, the citric acid cycle, and oxidative phosphorylation.
• Sirtuin and PARP Activity Assays: Serves as a labeled substrate for investigating the activity of NAD+-dependent enzymes like sirtuins and poly(ADP-ribose) polymerases (PARPs), which are key targets in aging and disease research.
• Drug Discovery & Screening: Employed in high-throughput screening assays to identify and characterize novel modulators of NAD+-utilizing enzymes for therapeutic development.
• Radioisotope Dilution Studies: Used in quantitative mass spectrometry and other analytical techniques for the accurate measurement of endogenous NAD+ pool sizes and turnover.
• Cell Signaling Studies: Facilitates research into calcium signaling and other cellular processes where NAD+ acts as a precursor for secondary messengers.

Basic Information

Product Name	Nicotinamide Adenine Dinucleotide, [Adenine-2,8-3H]
CAS No.	107688-05-1
Molecular Formula	C21H27N7O14P2 * (³H)2
Molecular Weight	~665.4 g/mol (unlabeled)
Synonyms	NAD+, [³H]; [2,8-³H]NAD+; β-NAD+, [adenine-2,8-³H]; Coenzyme I, Tritiated; DPN+, Tritiated; Oxidized Nicotinamide Adenine Dinucleotide, Tritiated; 1,4-Dihydronicotinamide adenine dinucleotide (oxidized form), [³H]; β-Nicotinamide adenine dinucleotide, [2,8-³H]adenine-labeled
EINECS	Contact for details

Quality Control

This high-purity radiolabeled biochemical is produced and handled under strict quality control protocols to ensure isotopic and chemical integrity. Each batch is accompanied by a comprehensive Certificate of Analysis (COA) detailing radiochemical purity (typically >97% by HPLC), specific activity (e.g., 20-40 Ci/mmol), chemical purity, and solvent composition. Our quality systems are designed to meet the exacting standards of research and pharmaceutical development.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store frozen at or below -20°C in the original solvent as supplied. Avoid repeated freeze-thaw cycles. This product is light-sensitive (store away from light). All handling must comply with local regulations for radioactive materials.

Specification

Item	Specification
Appearance	Clear, colorless to slightly yellow solution
Identification (HPLC)	Conforms to reference standard
Radiochemical Purity (HPLC)	≥ 97%
Specific Activity	20 - 40 Ci/mmol (Contact for exact value)
Chemical Purity (HPLC, UV)	≥ 95%
Solvent	Ethanol:Water mixture (specified on COA)
Concentration	1 mCi/mL (approx.)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.