Description

5-Bromo-4-Chloro-3-Indoxyl Phosphate, Bis(2-Amino-2-Methyl-1,3-Propanediol) Salt is a high-purity chromogenic substrate essential for enzymatic detection systems. This compound is critical for researchers and diagnostic manufacturers requiring precise, sensitive, and reliable colorimetric assays. It is primarily utilized in molecular biology, histochemistry, and immunodiagnostics for the detection of alkaline phosphatase activity.

Application

• Chromogenic Substrate for Alkaline Phosphatase (AP): Used in ELISA, Western blotting, and immunohistochemistry (IHC) for generating an insoluble blue indigo dye precipitate.
• Molecular Biology Research: Employed in nucleic acid hybridization techniques, such as Southern and Northern blotting, for non-radioactive detection.
• Diagnostic Kit Manufacturing: A key component in the production of clinical diagnostic kits for various biomarkers and infectious diseases.
• Cell and Tissue Staining: Facilitates the localization of AP-tagged antibodies or probes in histological samples.
• Microbiology: Used in assays for detecting specific bacterial enzymes or in phage display technology.
• Quality Control (QC) Testing: Applied in biopharmaceutical QC for verifying the presence or activity of AP-conjugated reagents.

Basic Information

Product Name	5-Bromo-4-Chloro-3-Indoxyl Phosphate, Bis(2-Amino-2-Methyl-1,3-Propanediol) Salt
CAS No.	107475-11-6
Molecular Formula	C17H28BrClN2O8P
Molecular Weight	528.74 g/mol
Synonyms	BCIP, 5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt (common misnomer), 5-Bromo-4-chloro-3-indoxyl phosphate disodium salt (related compound), BCIP salt, BCIP substrate, Alkaline Phosphatase Substrate BCIP, 3-Indolyl phosphate, 5-bromo-4-chloro-, bis(2-amino-2-methyl-1,3-propanediol) salt, X-Phosphate
EINECS	Contact for details

Quality Control

Our 5-Bromo-4-Chloro-3-Indoxyl Phosphate, Bis(2-Amino-2-Methyl-1,3-Propanediol) Salt is manufactured under strict quality systems to ensure batch-to-batch consistency and high performance in sensitive applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and suitability for use as a chromogenic substrate. We adhere to relevant industry guidelines to support your research and diagnostic requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. The product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMF, DMSO; slightly soluble in water
Functional Test (AP Assay)	Produces characteristic blue precipitate

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.